一种新型功能性兔白细胞介素-7 同工型。
A novel functional rabbit IL-7 isoform.
机构信息
Department of Microbiology and Immunology, Stritch School of Medicine, Loyola University Chicago, 2160 South First Avenue, Maywood, IL 60153, USA.
出版信息
Dev Comp Immunol. 2010 Aug;34(8):828-36. doi: 10.1016/j.dci.2010.03.003. Epub 2010 Mar 26.
IL-7 is required for B cell development in mouse and is a key regulator of T cell development and peripheral T cell homeostasis in mouse and human. Recently, we found that IL-7 is expressed in rabbit bone marrow and in vitro, is required for differentiation of lymphoid progenitors to B and T lineage cells. Herein, we report the identification of a novel rabbit IL-7 isoform, IL-7II. Recombinant IL-7II (rIL-7II) binds lymphocytes via the IL-7R and induces phosphorylation of STAT5. Further, rIL-7II supports proliferation and differentiation of BM progenitor cells into B and T lineage cells. IL7-II is generated by alternative splicing, with an 11 amino acid insertion encoded by a separate exon, exon 2b. Exon 2b is conserved in other lagomorphs, in Perissodactyla, Artiodactyla, and Carnivora, but is absent in mouse and human.
IL-7 是小鼠 B 细胞发育所必需的，也是调节小鼠和人类 T 细胞发育和外周 T 细胞稳态的关键因子。最近，我们发现 IL-7 在兔骨髓中表达，并且在体外，它对于淋巴祖细胞向 B 和 T 细胞谱系细胞的分化是必需的。在此，我们报道了一种新型兔 IL-7 同工型 IL-7II 的鉴定。重组 IL-7II(rIL-7II)通过 IL-7R 结合淋巴细胞，并诱导 STAT5 磷酸化。此外，rIL-7II 支持 BM 祖细胞增殖和分化为 B 和 T 细胞谱系细胞。IL7-II 通过选择性剪接产生，由一个单独的外显子编码 11 个氨基酸插入，外显子 2b。外显子 2b 在其他兔形目动物、奇蹄目、偶蹄目和食肉目动物中保守，但在小鼠和人类中缺失。
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