College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing High-tech Industrial Development Zone, Daqing, PR China.
Anaerobe. 2010 Aug;16(4):317-20. doi: 10.1016/j.anaerobe.2010.03.007. Epub 2010 Mar 19.
A serodiagnostic ELISA (rL-ELISA) using recombinant truncated leukotoxin protein PL2 (aa 311-644) of Fusobacterium necrophorum as antigen was developed for detection of antibodies against F. necrophorum from cattle footrot. In rL-ELISA, the recombinant diagnostic antigen showed no cross-reaction with antisera against bovine foot and mouth disease virus, bovine rhinotracheitis virus, bovine viral diarrhea virus, bovine rotavirus type A, bovine Escherichia coli, and bovine Salmonella. The rL-ELISA could confirm the existence of antibodies against F. necrophorum at day 7 after infection. Detection of the field samples indicated relative sensitivity of rL-ELISA to nL-ELISA using the purified native leukotoxin A as antigen was 96.43%, and relative specificity of rL-ELISA to nL-ELISA was 94.26%. These data demonstrated the rL-ELISA would have a potential use for early diagnosis of cattle footrot caused by F. necrophorum.
采用重组截短白细胞毒素蛋白 PL2（aa311-644）作为抗原的血清学酶联免疫吸附试验（rL-ELISA），用于检测牛腐蹄病中抗坏死梭杆菌的抗体。在 rL-ELISA 中，重组诊断抗原与抗牛口蹄疫病毒、牛鼻气管炎病毒、牛病毒性腹泻病毒、牛轮状病毒 A 型、牛大肠杆菌和牛沙门氏菌的血清无交叉反应。rL-ELISA 可在感染后第 7 天确认抗坏死梭杆菌抗体的存在。对现场样本的检测表明，rL-ELISA 的相对敏感性比使用纯化天然白细胞毒素 A 作为抗原的 nL-ELISA 高 96.43%，rL-ELISA 的相对特异性比 nL-ELISA 高 94.26%。这些数据表明，rL-ELISA 可用于早期诊断由坏死梭杆菌引起的牛腐蹄病。
