Tsuruya T, Hata K, Kamimura T, Hayakawa A, Aoyagi Y, Asakura H, Ishihara K, Toshima M, Soga K, Sibasaki K
Third Department of Internal Medicine, Niigata University School of Medicine, Japan.
Nihon Shokakibyo Gakkai Zasshi. 1991 Feb;88(2):131-7.
The sera from patients with various liver diseases were investigated for the antibody against calmodulin (CaM) extracted from bovine brain by the enzyme linked immunosorbent assay. The specificity and purity of CaM were confirmed by the Western blot technique using anti-CaM antibody (anti-CaM) positive sera. IgA class antibody was frequently detected in patients with hepatocellular carcinoma (HCC), autoimmune hepatitis (AIH) and chronic active hepatitis (CAH). On the other hand, IgG class antibody was very often present in patients liver cirrhosis, AIH and acute viral hepatitis (AVH). Sixty seven percent of patients with AVH in the acute phase were positive for IgM class anti-CaM and 33% of patients with AVH in the convalescent phase positive respectively. In AVH, the titer of anti-CaM reached its peak on 26.3 days after the onset. The titer of anti-CaM in fulminant hepatitis was higher than that in AVH. Seventy percent of type A hepatitis patients were positive for IgM class anti-CaM, 33% of type B and 33% of type non-A non-B. These results suggest that the frequency and titer of anti-CaM may depend upon the type of hepatitis and the degree of liver cell injury.
采用酶联免疫吸附试验，对患有各种肝脏疾病患者的血清进行检测，以查找针对从牛脑中提取的钙调蛋白（CaM）的抗体。使用抗钙调蛋白抗体（抗CaM）阳性血清，通过蛋白质印迹技术确认了CaM的特异性和纯度。在肝细胞癌（HCC）、自身免疫性肝炎（AIH）和慢性活动性肝炎（CAH）患者中，经常检测到IgA类抗体。另一方面，在肝硬化、AIH和急性病毒性肝炎（AVH）患者中，IgG类抗体很常见。急性期67%的AVH患者IgM类抗CaM呈阳性，恢复期33%的AVH患者分别呈阳性。在AVH中，抗CaM滴度在发病后26.3天达到峰值。暴发性肝炎中抗CaM滴度高于AVH。70%的甲型肝炎患者IgM类抗CaM呈阳性，乙型肝炎患者为33%，非甲非乙型肝炎患者为33%。这些结果表明，抗CaM的频率和滴度可能取决于肝炎类型和肝细胞损伤程度。
