Instituto de Medicina Tropical Alexander von Humboldt, Universidad Peruana Cayetano Heredia, Av. Honorio Delgado 430, San Martin de Porras, Lima 33, Peru.
Biometals. 2010 Jun;23(3):515-21. doi: 10.1007/s10534-010-9325-1. Epub 2010 Mar 21.
Lactoferrin (LF) has in vitro antimicrobial activity against Gram-negative bacteria. Salmonella enterica subsp. enterica serovar Typhimurium causes systemic infection and acute diarrhea in humans, mainly in children younger than 2 years of age. The aim of the study was to determine the in vivo effect of bovine LF in Salmonella ser. Typhimurium infection in mice. 58 BALB/c mice were employed. Two hours before the infection with 300 microl of 10(7) CFU of Salmonella ser. Typhimurium, 29 mice received LF (2 mg) and 29 placebo (buffer). After the infection, the mice received LF (10 mg/ml) ad libitum or buffer, respectively, for 7 days. Mortality, weight and clinical signs (piloerection, hunched position and reduced movement) were monitored daily. The degree of inflammation and necrosis in the intestine, liver, spleen and brain were studied with a blinded observer. The mortality in the control group (8/29) was higher than in the LF group (1/29) (Kapplan Meier P < 0.05). From the third day post-infection the control group were significantly more symptomatic (P < 0.05). The blood culture for Salmonella spp. was positive for all mice studied in the control group (17/17), but positive in the LF group in only 6/17 animals (P < 0.05). In the LF group, the pathologic studies show less inflammation and focal necrosis in the four organs studied, with the greatest difference found in the intestine. Bovine LF protects against Salmonella ser. Typhimurium infection in mice, reducing the severity, mortality and the degree of inflammation of this infection.
乳铁蛋白（LF）具有体外抗革兰氏阴性菌的活性。肠炎沙门氏菌亚种。肠炎血清型鼠伤寒引起全身性感染和急性腹泻，主要发生在 2 岁以下儿童。本研究旨在确定牛 LF 在鼠伤寒沙门氏菌感染中的体内作用。采用 58 只 BALB/c 小鼠。在感染前 2 小时，用 300 微升 10（7）CFU 的鼠伤寒沙门氏菌感染 29 只小鼠，29 只接受 LF（2mg）和 29 只安慰剂（缓冲液）。感染后，分别给感染的小鼠自由饮用 LF（10mg/ml）或缓冲液，持续 7 天。每天监测死亡率、体重和临床症状（毛发竖立、缩背和运动减少）。用盲法观察者研究肠道、肝脏、脾脏和大脑的炎症和坏死程度。对照组（8/29）的死亡率高于 LF 组（1/29）（Kapplan Meier P < 0.05）。从感染后第三天起，对照组的症状明显更为严重（P < 0.05）。对照组所有研究小鼠的血培养均为沙门氏菌阳性（17/17），但 LF 组仅 6/17 动物阳性（P < 0.05）。在 LF 组，病理研究显示，在四个研究器官中，炎症和局灶性坏死程度较低，其中肠道差异最大。牛 LF 可预防鼠伤寒沙门氏菌感染小鼠，减轻感染的严重程度、死亡率和炎症程度。
