Kunming Center for Disease Control and Prevention, No. 4, Ziyun Road, Kunming 650228, China.
Toxicol In Vitro. 2010 Jun;24(4):1119-25. doi: 10.1016/j.tiv.2010.03.008. Epub 2010 Mar 20.
Gasoline engine exhaust has been considered as a major source of air pollution in China. Due to lower cyto- and geno-toxicity effects of methanol engine exhaust, methanol is regarded as a potential substitute for gasoline. We have previously compared cyto- and geno-toxicities of gasoline engine exhaust with that of methanol engine exhaust in A549 cells (Zhang et al., 2007).To characterize the immunotoxic effects for gasoline and methanol engine exhausts in immune cell, in this study, we further compared effects of gasoline and methanol engine exhausts on immune function in RAW264.7 cell and rabbit alveolar macrophages. Results showed that both gasoline and methanol engine exhaust could evidently inhibit RAW264.7 cell proliferation, promote RAW264.7 cell apoptosis, decrease E-rosette formation rate and inhibit anti-tumor effects of alveolar macrophages, at the same time, these effects of gasoline engine exhaust were far stronger than those of methanol engine exhaust. In addition, gasoline engine exhaust could significantly inhibit activities of ADCC of alveolar macrophages, but methanol engine exhaust could not. These results suggested that both gasoline and methanol engine exhausts might be immunotoxic atmospheric pollutants, but some effects of gasoline engine exhaust on immunotoxicities may be far stronger than that of methanol engine exhaust.
汽油发动机尾气已被认为是中国空气污染的主要来源。由于甲醇发动机尾气的细胞毒性和遗传毒性较低，甲醇被认为是汽油的潜在替代品。我们之前已经比较了 A549 细胞中汽油发动机尾气和甲醇发动机尾气的细胞毒性和遗传毒性（Zhang 等人，2007 年）。为了描述汽油和甲醇发动机尾气对免疫细胞的免疫毒性作用，在本研究中，我们进一步比较了汽油和甲醇发动机尾气对 RAW264.7 细胞和兔肺泡巨噬细胞免疫功能的影响。结果表明，汽油和甲醇发动机尾气均可明显抑制 RAW264.7 细胞增殖，促进 RAW264.7 细胞凋亡，降低 E-玫瑰花结形成率，抑制肺泡巨噬细胞的抗肿瘤作用，同时，汽油发动机尾气的这些作用明显强于甲醇发动机尾气。此外，汽油发动机尾气可显著抑制肺泡巨噬细胞的 ADCC 活性，但甲醇发动机尾气则不能。这些结果表明，汽油和甲醇发动机尾气均可能是具有免疫毒性的大气污染物，但汽油发动机尾气对免疫毒性的某些作用可能明显强于甲醇发动机尾气。
