Noguchi I, Arai H, Iizuka R
Department of Psychiatry, Juntendo University School of Medicine, Tokyo, Japan.
J Neural Transm Gen Sect. 1991;83(3):171-8. doi: 10.1007/BF01253387.
Postmortem stability of arginine-vasopressin (AVP) messenger RNA (mRNA) in the rat brain was studied comparing changes with those in the recovered amounts of total RNA and ribosomal RNA (rRNA). The amount of AVP mRNA and rRNA showed a decrease with increasing time interval after death (postmortem time), whereas the amount of total RNA did not alter with postmortem time. The half-life of AVP mRNA in the rat postmortem seemed to be approximately 16 hrs. The analysis of the ratio of AVP mRNA to 18S-rRNA suggested that AVP mRNA was degraded postmortem more rapidly than rRNA. These results suggest that autopsied human brains should be used for AVP mRNA study within a short postmortem time.
通过比较大鼠脑中精氨酸加压素（AVP）信使核糖核酸（mRNA）与总RNA和核糖体RNA（rRNA）回收量的变化，研究了其死后稳定性。死后时间间隔（尸检时间）增加时，AVP mRNA和rRNA的量减少，而总RNA的量不随尸检时间改变。大鼠死后AVP mRNA的半衰期似乎约为16小时。AVP mRNA与18S-rRNA比例的分析表明，死后AVP mRNA比rRNA降解得更快。这些结果表明，尸检后的人类大脑应在短尸检时间内用于AVP mRNA研究。
