Bando K, Schueler S, Cameron D E, DeValeria P A, Hatanaka M, Casale A S, Zebley M A, Hutchins G M, Reitz B A, Baumgartner W A
Division of Cardiac Surgery, Johns Hopkins Medical Institution, Baltimore, Md.
J Heart Lung Transplant. 1991 Mar-Apr;10(2):304-9.
Because leukocytes and oxygen radical species contribute to ischemic and reperfusion injury during organ preservation, we examined the effects of a long-acting liposomal superoxide dismutase (liposomal SOD) and mechanical filtration of leukocytes on cardiopulmonary graft function after 12 hours of static preservation. Bovine heart-lung blocks were harvested, core cooled to 15 degrees C, stored in 4 degrees C donor blood for 12 hours, and then orthotopically transplanted (control group, n = 6). In the leukocyte-depletion group (n = 6), a leukocyte filter was incorporated in the bypass circuits of the donor and recipient. In the SOD group (n = 6), liposomal SOD (5000 U/kg) was administered in the cardioplegic solution, in the prime of the bypass circuits of donor and recipient, and immediately before recipient heart-lung reperfusion. In the combination group (n = 6), both leukocyte depletion (LD) and liposomal SOD were used. Only four of six control animals survived more than 2 hours after weaning from bypass, whereas all LD, SOD, and LD + SOD animals survived to be studied at 6 hours. Pulmonary function was assessed at 6 hours by arterial oxygen tension on 100% inspired oxygen (PO2), pulmonary vascular resistance (PVR), and postmortem wet/dry lung weight ratios. Arterial pO2 values (mm Hg) were as follows: control, 102 +/- 51; LD, 437 +/- 60*; SOD, 278 +/- 83; and LD + SOD, 504 +/- 54* (p less than 0.05 vs controls). PVR values (dynes . sec . cm5) were as follows: control, 1975 +/- 697; LD, 682 +/- 131; SOD, 607 +/- 191*; and LD + SOD 367 +/- 87* (*p less than 0.05 vs controls).(ABSTRACT TRUNCATED AT 250 WORDS)
由于白细胞和氧自由基在器官保存过程中会导致缺血再灌注损伤，我们研究了长效脂质体超氧化物歧化酶（脂质体SOD）以及白细胞机械过滤对静态保存12小时后心肺移植物功能的影响。采集牛心肺块，核心温度冷却至15℃，在4℃供体血液中保存12小时，然后进行原位移植（对照组，n = 6）。在白细胞清除组（n = 6）中，在供体和受体的旁路循环中加入白细胞滤器。在SOD组（n = 6）中，脂质体SOD（5000 U/kg）在心脏停搏液中、供体和受体旁路循环预充时以及受体心肺再灌注前即刻给药。在联合组（n = 6）中，同时使用白细胞清除（LD）和脂质体SOD。对照组6只动物中只有4只在脱离旁路后存活超过2小时，而所有LD组、SOD组和LD + SOD组动物均存活至6小时进行研究。在6小时时通过吸入100%氧气时的动脉血氧分压（PO2）、肺血管阻力（PVR）以及死后肺湿/干重比评估肺功能。动脉pO2值（mmHg）如下：对照组，102±51；LD组，437±60*；SOD组，278±83；LD + SOD组，504±54*（与对照组相比p < 0.05）。PVR值（达因·秒·cm5）如下：对照组，1975±697；LD组，682±131；SOD组，607±191*；LD + SOD组367±87*（*与对照组相比p < 0.05）。（摘要截断于250字）
