Mann B, Grayeski M L
Department of Chemistry, Seton Hall University, South Orange, NJ 07079.
Biomed Chromatogr. 1991 Jan;5(1):47-52. doi: 10.1002/bmc.1130050111.
Peroxyoxalate chemiluminescence may be used for sensitive postcolumn detection of phenothiazine analytes separated by high performance liquid chromatography with appropriate optimization of measurement conditions such as solvent, pH and oxalate ester. Detectability of fluorescent analytes by chemical excitation varies greatly, but analytes with low oxidation potentials are generally more readily detected at low levels, as demonstrated for phenothiazines, an important class of fluorescent drugs. Some improvement in detection limits is observed for fluphenazine when chemiluminescence detection is compared to conventional fluorescence detection. Because of the specificity of chemical excitation, fewer interferences from fluorescent impurities in a urine matrix are observed.
过氧草酸酯化学发光可用于高效液相色谱分离的吩噻嗪分析物的柱后灵敏检测，需对测量条件如溶剂、pH值和草酸酯进行适当优化。通过化学激发对荧光分析物的可检测性差异很大，但氧化电位低的分析物通常在低水平时更容易被检测到，如吩噻嗪类所示，这是一类重要的荧光药物。与传统荧光检测相比，当采用化学发光检测时，氟奋乃静的检测限有一定改善。由于化学激发的特异性，在尿液基质中观察到的来自荧光杂质的干扰较少。
