Bühring H J, Saalmüller A, Müller C, van Agthoven A J, Busch F W
Second Department of Internal Medicine, Medical University Clinic of Tübingen, Federal Republic of Germany.
Hybridoma. 1991 Feb;10(1):77-88. doi: 10.1089/hyb.1991.10.77.
A monoclonal antibody (11G7) detecting a novel antigen on human hemopoietic progenitor cells (named 11G7R = 11G7 receptor) was raised by immunization of a Balb/c mouse with the leukemic blasts of a patient suffering from chronic myelogenous leukemia blast crisis (CML-BC). The antigen is expressed on most of MHC class II bearing peripheral blood leucocytes (PBL) and on a subpopulation of bone marrow mononuclear cells (BMMNC). By FACS-sorting and colony assays, it could be demonstrated that 11G7R is expressed on myelo-monocytic and myelo-granulocytic bone marrow precursor cells (GM-CFC, G-CFC, M-CFC) but is absent from erythroid precursor cells (BFU-E) and on cells exhibiting the capacity to form mixed colonies (GEMM-CFC). Double-fluorescence analysis on BMMNC revealed that 11G7R is expressed on a subset of B-cells, myeloid cells and cells carrying the HPCA-1 antigen (CD34). It has a similar distribution pattern to the myeloid antigens CD13 and CD33. However, in contrast to these antigens, 11G7R is also expressed on the blasts of several lymphoid leukemias (4/9 B-ALL, 1/2 T-ALL) and therefore it is not restricted to the myeloid lineage.
用一名慢性粒细胞白血病急变期（CML-BC）患者的白血病原始细胞免疫Balb/c小鼠，制备出一种单克隆抗体（11G7），该抗体可检测人类造血祖细胞上的一种新抗原（命名为11G7R = 11G7受体）。该抗原在大多数表达MHC II类分子的外周血白细胞（PBL）以及一部分骨髓单个核细胞（BMMNC）上表达。通过荧光激活细胞分选术（FACS）和集落分析可以证明，11G7R在骨髓粒-单核细胞和粒-粒细胞前体细胞（GM-CFC、G-CFC、M-CFC）上表达，但在红系前体细胞（BFU-E）以及具有形成混合集落能力的细胞（GEMM-CFC）上不表达。对BMMNC进行双荧光分析显示，11G7R在一部分B细胞、髓系细胞以及携带HPCA-1抗原（CD34）的细胞上表达。它与髓系抗原CD13和CD33具有相似的分布模式。然而，与这些抗原不同的是，11G7R也在几种淋巴白血病的原始细胞上表达（4/9 B-ALL、1/2 T-ALL），因此它并不局限于髓系谱系。
