Ranck Martin Frederick, Schmidt Volker, Philipp Hans-Christian, Voss Matthias, Kacza Johannes, Richter Andreas, Fehlhaber Karsten, Krautwald-Junghanns Maria-Elisabeth
Klinik für Vögel und Reptilien der Universität Leipzig.
Berl Munch Tierarztl Wochenschr. 2010 Mar-Apr;123(3-4):111-8.
Hens laying eggs with egg-pole shell defects (EPS) were examined in a clinical prospective study. 86 hens with EPS and 72 hens without EPS from three flocks were selected for this study. It could be proven serologically that hens with EPS had significant (p < 0.05) higher titers against Mycoplasma (M.) synoviae then hens without EPS. PCR tested cloacal swabs for M. synoviae were more frequently positive from hens with EPS (87%; n=72) then from hens without EPS (18%; n=13). Furthermore, M. synoviae could be cultivated from the oviduct of five hens with EPS. Additionally, M. synoviae-DNA was detectable in the albumen of nearly all eggs with EPS (n=48; 98%), contrary to the eggs without EPS (n=11; 26%). Ultrastructural investigation revealed that eggs with EPS showed considerable differences of the egg shell structure as well as the cross section dimension according to eggs without EPS. Due to the significantly more frequent detection of M. synoviae-DNA from the cloaca of chickens with EPS, is an involvement of M. synoviae in laying of EPS in the surveyed herds likely. Further infection experiments with the isolated M. synoviae were not perfomed, therefore about the causal pathogenic role of M. synoviae in the development of eggs with EPS in the surveyed herds can only be speculated.
在一项临床前瞻性研究中，对产蛋出现蛋极壳缺陷（EPS）的母鸡进行了检查。从三个鸡群中挑选了86只患有EPS的母鸡和72只未患EPS的母鸡用于本研究。血清学检测证实，患有EPS的母鸡针对滑液支原体（M. synoviae）的抗体效价显著（p < 0.05）高于未患EPS的母鸡。对滑液支原体进行PCR检测时，患有EPS的母鸡泄殖腔拭子呈阳性的频率（87%；n = 72）高于未患EPS的母鸡（18%；n = 13）。此外，从五只患有EPS的母鸡的输卵管中培养出了滑液支原体。另外，几乎所有患有EPS的鸡蛋（n = 48；98%）的蛋清中都可检测到滑液支原体DNA，而未患EPS的鸡蛋（n = 11；26%）则不然。超微结构研究表明，与未患EPS的鸡蛋相比，患有EPS的鸡蛋在蛋壳结构以及横截面尺寸方面存在显著差异。由于从患有EPS的鸡的泄殖腔中检测到滑液支原体DNA的频率明显更高，因此滑液支原体很可能与所调查鸡群中EPS的产生有关。未对分离出的滑液支原体进行进一步的感染实验，因此只能推测滑液支原体在所调查鸡群中EPS鸡蛋形成过程中的致病作用。
