Venugopalan C S, Holmes E P, Fucci V, Guthrie A J
Department of Veterinary Physiology, School of Veterinary Medicine, Louisiana State University, Baton Rouge 70803.
J Appl Physiol (1985). 1991 Mar;70(3):1006-10. doi: 10.1152/jappl.1991.70.3.1006.
We examined the inhibitory and excitatory components of the nonadrenergic noncholinergic (NANC) innervation of the guinea pig airways by in vivo and in vitro methods. Electrical stimulation of the vagus in chloralose-urethan-anesthetized guinea pigs after cholinergic and adrenergic blockade produced peripheral airway constriction (insufflation pressure) and tracheal relaxation (pouch pressure). Vagal stimulation was applied for 90 s at 5-V pulses of 2-ms duration at frequencies of 5, 15, 25, and 35 Hz in each group (n = 6). The pouch relaxation peaked at 15 Hz. The insufflation pressure was highest at 5 Hz. Field stimulations of the same frequencies were applied on tracheal spirals and lung parenchymal strips. The maximal relaxation of the trachea occurred at 15-35 Hz. The lung parenchymal strip tensions increased almost linearly as the frequency increased from 5 to 35 Hz. The results of the study indicated a frequency-dependent response for both excitatory and inhibitory components of the NANC, which operate at different frequencies for optimal responses.
我们通过体内和体外方法研究了豚鼠气道非肾上腺素能非胆碱能（NANC）神经支配的抑制性和兴奋性成分。在胆碱能和肾上腺素能阻断后，对氯醛糖-乌拉坦麻醉的豚鼠迷走神经进行电刺激，可引起外周气道收缩（吹入压力）和气管舒张（气囊压力）。每组（n = 6）以5、15、25和35 Hz的频率，施加持续时间为2 ms、电压为5 V的脉冲，对迷走神经进行90 s的刺激。气囊舒张在15 Hz时达到峰值。吹入压力在5 Hz时最高。对气管螺旋条和肺实质条施加相同频率的场刺激。气管的最大舒张发生在15 - 35 Hz。随着频率从5 Hz增加到35 Hz，肺实质条的张力几乎呈线性增加。研究结果表明，NANC的兴奋性和抑制性成分均存在频率依赖性反应，它们在不同频率下发挥作用以实现最佳反应。
