Kazan Institute of Biochemistry and Biophysics, Kazan Scientific Center of the Russian Academy of Sciences, Kazan, 420111, Russia.
Biochemistry (Mosc). 2010 Jan;75(1):95-100. doi: 10.1134/s0006297910010128.
Infiltration of wheat (Triticum aestivum L.) seedling leaves with excess of nitrate, nitrite, or the NO donor sodium nitroprusside leads to increase both in content of hydroperoxide and activity of peroxidase and decrease in superoxide dismutase (SOD) activity in the leaf apoplast. Polymorphism of extracellular peroxidases and the presence of Cu/Zn-SOD have been shown in apoplast. Using an ESR assay, a considerable increase in the level of NO following infiltration of leaf tissues with nitrite has been demonstrated. These data suggest development of both oxidative and nitrosative stresses in leaves exposed to high levels of nitrate or nitrite. A possible interplay of NO and reactive oxygen species in plant cells is discussed.
过量的硝酸盐、亚硝酸盐或一氧化氮供体硝普酸钠渗透到小麦(Triticum aestivum L.)幼苗叶片中,会导致叶片质外体中超氧化物歧化酶(SOD)活性的降低和过氧化物含量及过氧化物酶活性的增加。已经证明,质外体中存在细胞外过氧化物酶的多态性和 Cu/Zn-SOD。通过 ESR 测定法,已经证明在将亚硝酸盐渗透到叶片组织后,NO 的水平会显著增加。这些数据表明,暴露于高浓度硝酸盐或亚硝酸盐的叶片中会产生氧化和硝化应激。讨论了植物细胞中 NO 和活性氧之间可能的相互作用。