Wang Xian-Ye, Shen Wen-Biao, Xu Lang-Lai
College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China.
Zhi Wu Sheng Li Yu Fen Zi Sheng Wu Xue Xue Bao. 2004 Apr;30(2):195-200.
When wheat (Triticum aestivum L. Yangmai 158) seedling (with three fully expanded leaves) roots were treated with 15% PEG-6000 in combination with different concentrations (0.1 and 0.5 mmol/L) of exogenous nitric oxide donor sodium nitroprusside (SNP) and NO(-)(3)/NO(-)(2) (control), the enhancement of lipoxygenase (LOX) activity in wheat seedling leaves under osmotic stress was delayed at the lower concentration of SNP treatment (0.1 mmol/L), while the generation rate of O(-.)(2), the enhancement of membrane permeability and the accumulation of MDA and H(2)O(2)were also alleviated. SNP 0.1 mmol/L significantly enhanced the activity of superoxide dismutase (SOD), and accelerated the accumulation of proline. In comparison with these changes, the effects of higher concentration of SNP (0.5 mmol/L) and corresponding control (NO(-)(3)/NO(-)(2)) were not so obvious. The above results indicate that lower concentrations of in vitro nitric oxide (NO) can protect wheat seedling leaves from membrane lipid peroxidation caused by osmotic stress.
用15%聚乙二醇-6000（PEG-6000）分别与不同浓度（0.1和0.5 mmol/L）的外源一氧化氮供体硝普钠（SNP）及硝酸盐/亚硝酸盐（对照）处理小麦（普通小麦扬麦158）幼苗（具3片完全展开叶）根系时，较低浓度SNP处理（0.1 mmol/L）延缓了渗透胁迫下小麦幼苗叶片脂氧合酶（LOX）活性的增强，同时超氧阴离子（O₂⁻·）生成速率、膜透性增强以及丙二醛（MDA）和过氧化氢（H₂O₂）积累也得到缓解。0.1 mmol/L的SNP显著增强了超氧化物歧化酶（SOD）活性，并加速了脯氨酸积累。与这些变化相比，较高浓度SNP（0.5 mmol/L）及相应对照（硝酸盐/亚硝酸盐）的影响不那么明显。上述结果表明，较低浓度的外源一氧化氮（NO）可保护小麦幼苗叶片免受渗透胁迫诱导的膜脂过氧化作用。
