Wenisch S, Arnhold S
Institute of Veterinary Anatomy, -Histology and -Embryology, Justus-Liebig-University, Giessen, 35392 Giessen, Germany.
Anat Histol Embryol. 2010 Jun;39(3):201-6. doi: 10.1111/j.1439-0264.2010.00996.x. Epub 2010 Mar 22.
NADPH-diaphorase (NADPH-d) staining of the bovine olfactory epithelium was compared with the immunohistochemical localization of nitric oxide synthase (NOS), soluble guanylyl cyclase, and cGMP (cyclic guanosine 3',5'-monophosphate). Out of the three isoforms, only the inducible NOS (NOS-II) was found at the epithelial surface correlating with the strong labelling for NADPH-d. In contrast, light diaphorase staining associated with deeper epithelial regions did not coincide with any NOS immunoreactivity. As there is overlapping expression of NOS-II, soluble guanylyl cyclase and cGMP at the luminal surface morphologically occupied by dendritic knobs of olfactory receptor neurons and microvillar endings of supporting cells, the nitric oxide (NO)/cGMP pathway is likely to be involved in modulating the odour signals during olfactory transduction.
对牛嗅觉上皮的还原型辅酶Ⅱ黄递酶（NADPH-d）染色结果与一氧化氮合酶（NOS）、可溶性鸟苷酸环化酶和环磷酸鸟苷（cGMP）的免疫组织化学定位进行了比较。在三种同工型中，仅诱导型NOS（NOS-II）在上皮表面被发现，且与NADPH-d的强染色相关。相反，与上皮深层区域相关的浅黄递酶染色与任何NOS免疫反应性均不相符。由于在嗅觉受体神经元的树突状小体和支持细胞的微绒毛末端在形态上占据的腔表面存在NOS-II、可溶性鸟苷酸环化酶和cGMP的重叠表达，一氧化氮（NO）/cGMP途径可能参与嗅觉转导过程中气味信号的调节。
