Department of Neuroscience and Pharmacology, The Panum Institute, University of Copenhagen, Copenhagen, Denmark.
Brain Res. 2010 May 17;1331:58-73. doi: 10.1016/j.brainres.2010.03.056. Epub 2010 Mar 21.
The present study aimed at characterizing the anatomical and subcellular localization of cytoglobin (Cygb) and neuroglobin (Ngb) in the mouse brain by use of in situ hybridisation, immunohistochemistry and immunoelectron microscopy. Cygb and Ngb were only found in distinct brain areas and often in the same areas. We found intense staining in the piriform cortex, amygdala, hypothalamus (medial preoptic area, supra chiasmatic nucleus, lateral hypothalamus (LH), ventromedial hypothalamic nucleus, and the arcuate nucleus, habenular nuclei, laterodorsal tegmental nucleus (LDTg), pedunculopontine tegmental nucleus (PPTg), locus coeruleus, nucleus of the solitary tract and the spinal trigeminal nucleus. In addition Cygb is found in the hippocampus, the reticular thalamic nucleus, and the dorsal raphe nucleus; Ngb is found in the sub parabrachial nucleus. Co-localization of Cygb and Ngb is mainly observed in the LDTg and PPTg. Cygb and Ngb were found in cytoplasm, along neurotubuli, in mitochondria and in the nucleus by use of immunoelectron microscopy. Most neuronal nitric oxide synthase (nNOS)-positive neurons were found to co-localize Cygb, although not all nNOS neurones contain Cygb. Ngb co-localize with almost all orexin neurons in the LH. In conclusion the distribution of Cygb and Ngb seems much more restricted and coherent than previously reported. We believe other functions than pure oxygen buffers and neuroprotectants should be considered. The anatomical data indicate a role in NO signalling for Cygb and involvement in sleep-wake cycling for Cygb and Ngb.
本研究旨在通过原位杂交、免疫组织化学和免疫电镜观察，描述小鼠脑内细胞色素 c 氧合酶（Cygb）和神经球蛋白（Ngb）的解剖和亚细胞定位。Cygb 和 Ngb 仅在特定脑区表达，且通常在同一脑区表达。我们发现，在梨状皮质、杏仁核、下丘脑（视前内侧核、视交叉上核、外侧下丘脑（LH）、腹内侧下丘脑核和弓状核、缰核、外侧背盖核（LDTg）、脚桥核被盖部（PPTg）、蓝斑核、孤束核和三叉神经脊束核中存在强烈的染色。此外，Cygb 还存在于海马、丘脑网状核和中缝背核；Ngb 存在于臂旁下核。Cygb 和 Ngb 的共定位主要观察到在 LDTg 和 PPTg。通过免疫电镜观察到 Cygb 和 Ngb 存在于细胞质中，沿着神经微管，存在于线粒体和细胞核中。大多数神经元型一氧化氮合酶（nNOS）阳性神经元与 Cygb 共定位，尽管并非所有 nNOS 神经元都含有 Cygb。Ngb 与 LH 中的几乎所有食欲素神经元共定位。总之，Cygb 和 Ngb 的分布似乎比以前报道的更局限和一致。我们认为，除了作为纯氧缓冲剂和神经保护剂外，它们可能具有其他功能。解剖学数据表明 Cygb 在一氧化氮信号转导中发挥作用，Cygb 和 Ngb 参与睡眠-觉醒循环。
