Institute for Clinical & Experimental Surgery, University of Saarland, Homburg/Saar, Germany.
Reprod Sci. 2010 Jun;17(6):568-77. doi: 10.1177/1933719110364417. Epub 2010 Mar 23.
Genistein has previously been shown to cause regression of endometriotic lesions. In the current study, we investigated whether this observation is due to the antiangiogenic activity of genistein. Endometrial fragments and ovarian follicles were transplanted into dorsal skinfold chambers of hamsters, which were treated with genistein (50 and 200 mg/kg) or vehicle (control). Vascularization and blood perfusion of the grafts was analyzed over 14 days using intravital fluorescence microscopy and histology. Genistein inhibited angiogenesis neither in endometriotic lesions nor in ovarian follicles. This was indicated by a final microvessel density of the grafts, which was comparable to that of controls. Blood perfusion was not affected by genistein treatment. At day 14 after transplantation, the grafts of genistein- and vehicle-treated animals exhibited a histomorphology, which was typical for well-vascularized endometriotic lesions and ovarian follicles without any signs of regression. Thus, genistein may not be considered for the development of antiangiogenic treatment strategies in the therapy of endometriosis.
染料木黄酮先前已被证明可导致子宫内膜异位病变的消退。在本研究中，我们研究了这种观察结果是否归因于染料木黄酮的抗血管生成活性。将子宫内膜碎片和卵巢卵泡移植到仓鼠的背部皮褶室中，并用染料木黄酮（50 和 200mg/kg）或载体（对照）处理。在 14 天内使用活体荧光显微镜和组织学分析移植物的血管生成和血液灌注。染料木黄酮既不能抑制子宫内膜异位病变中的血管生成，也不能抑制卵巢卵泡中的血管生成。这表明移植物的最终微血管密度与对照组相当。血液灌注不受染料木黄酮治疗的影响。在移植后 14 天，用染料木黄酮和载体处理的动物的移植物表现出典型的血管丰富的子宫内膜异位病变和卵巢卵泡的组织形态，没有任何消退的迹象。因此，在子宫内膜异位症的治疗中，染料木黄酮可能不被认为是用于开发抗血管生成治疗策略的药物。
