Tang Center for Herbal Medicine Research, and Department of Anesthesia & Critical Care, Pritzker School of Medicine, University of Chicago, Chicago, IL 60637, USA.
Anticancer Res. 2010 Feb;30(2):295-302.
In this study, we investigated the inhibitive effects of Oplopanax horridus extract (OhE) and its fractions (OhF1, OhF2, OhF3, OhF4 and OhF5) on the growth of human colorectal cancer cells and the possible mechanisms involved were investigated.
The antiproliferative effects were evaluated by MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) cell proliferation assay. Apoptotic effects and cell cycle distribution were analyzed by flow cytometry after staining with Annexin V/PI or PI/RNase.
After treatment for 48 h, OhE, OhF4 and OhF5 (10-100 microg/ml) inhibited proliferation of HCT-116, SW-480 and HT-29 cell lines, and cell growth decreased most with the treatment of OhF4. On the other hand, OhF1, OhF2 and OhF3 were not observed to have obvious suppressive effects on these cell lines at concentrations of 10-100 microg/ml. OhE, OhF4 and OhF5 (1-10 microg/ml) noticeably induced apoptosis time- and concentration-dependently compared to the control at the same time point. Treatment with OhE, OhF4 or OhF5 (1-10 microg/ml) for 24 h distinctly induced a G(2)/M-phase arrest of the cell cycle in a dose-dependent manner. The trend of increasing cyclin A and cyclin B1 were similar to the increase of G(2)/M phase cells in all treated groups.
These results showed that OhE had potential antiproliferative effects on human colorectal cancer cells, and the active components are enriched in the OhF4 and OhF5 fractions. The anticancer mechanism of OhE, OhF4 and OhF5 might be attributed to the induction of apoptosis and the regulation of cell cycle transition.
本研究旨在探讨朝鲜蓟提取物（OhE）及其各馏分（OhF1、OhF2、OhF3、OhF4 和 OhF5）对人结直肠癌细胞生长的抑制作用，并探讨其可能的作用机制。
采用 MTS（3-（4，5-二甲基噻唑-2-基）-5-（3-羧基甲氧基苯基）-2-（4-磺基苯基）-2H-四唑）细胞增殖试验评估增殖抑制作用。经 Annexin V/PI 或 PI/RNase 染色后，通过流式细胞术分析凋亡作用和细胞周期分布。
OhE、OhF4 和 OhF5（10-100μg/ml）处理 48 h 后，抑制 HCT-116、SW-480 和 HT-29 细胞系的增殖，其中 OhF4 处理时细胞生长下降最明显。另一方面，在 10-100μg/ml 浓度下，OhF1、OhF2 和 OhF3 对这些细胞系没有明显的抑制作用。与同一时间点的对照组相比，OhE、OhF4 和 OhF5（1-10μg/ml）时间和浓度依赖性地明显诱导细胞凋亡。OhE、OhF4 或 OhF5（1-10μg/ml）处理 24 h 后，细胞周期明显呈剂量依赖性地在 G2/M 期阻滞。所有处理组中环素 A 和环素 B1 的增加趋势与 G2/M 期细胞的增加相似。
这些结果表明，OhE 对人结直肠癌细胞具有潜在的增殖抑制作用，其活性成分在 OhF4 和 OhF5 馏分中富集。OhE、OhF4 和 OhF5 的抗癌机制可能归因于诱导细胞凋亡和调节细胞周期转换。
