Tang Center for Herbal Medicine Research, The Pritzker School of Medicine, University of Chicago, Chicago, IL 60637, USA.
Fitoterapia. 2010 Mar;81(2):132-9. doi: 10.1016/j.fitote.2009.08.003. Epub 2009 Aug 14.
Oplopanax horridus or devil's club is a herbal medicine distributed in North America. The constituents and pharmacological activities of O. horridus (OPH) are largely unknown. In this study, we assayed OPH stem and berry extracts using high performance liquid chromatography (HPLC). The anticancer potentials of extracts on different human cancer cell lines (SW-480, HCT-116, HT-29, MCF-7 and NSCLC) were determined by MTS method. The effect of stem extract on cancer cell cycle, expression of cyclin A, and apoptosis were assayed using flow cytometry. HPLC data showed that the composition of OPH stem extract is more complicated than the berry extract. The wavelength of maximum absorption of the major constituent in stem and berry is 196.0 nm and 201.9 nm, respectively. Compared to the berry extract, the stem extract showed significant potent antiproliferative effect on all the studied cell lines. The stem extract at 0.1 mg/ml arrested cancer cells in S- and G2/M-phases, and significantly induced expression of cyclin A. After treatment with 0.1 mg/ml of stem extract for 72 h, apoptotic cells were increased to 45.2%, while control was 9.6%. The cell cycle arrest and induction of apoptosis may play a critical role in cancer chemoprevention by Oplopanax horridus stem extract.
棘茎楤木（Oplopanax horridus）或恶魔之棒是一种分布于北美的草药。棘茎楤木（OPH）的成分和药理活性在很大程度上尚不清楚。在本研究中，我们使用高效液相色谱法（HPLC）对 OPH 茎和浆果提取物进行了分析。通过 MTS 法测定了提取物对不同人癌细胞系（SW-480、HCT-116、HT-29、MCF-7 和 NSCLC）的抗癌潜力。通过流式细胞术测定了茎提取物对癌细胞周期、细胞周期蛋白 A 表达和细胞凋亡的影响。HPLC 数据显示，OPH 茎提取物的成分比浆果提取物更为复杂。茎和浆果中主要成分的最大吸收波长分别为 196.0nm 和 201.9nm。与浆果提取物相比，茎提取物对所有研究的细胞系均表现出显著的增殖抑制作用。浓度为 0.1mg/ml 的茎提取物可将癌细胞阻滞在 S 和 G2/M 期，并显著诱导 cyclin A 的表达。用 0.1mg/ml 的茎提取物处理 72 小时后，凋亡细胞增加到 45.2%，而对照组为 9.6%。细胞周期阻滞和诱导凋亡可能在棘茎楤木茎提取物的癌症化学预防中发挥关键作用。
