Charité Campus Mitte, Institute of Pathology, Charitéplatz 1, D-10117 Berlin, Germany.
Anticancer Res. 2010 Feb;30(2):693-8.
Y-Box protein 1 (YB-1) is a multifunctional cellular protein expressed in a range of mammalian cells, including human cancer cells. It is involved in the regulation of various genes including cancer-associated genes, but the full range of target genes and regulatory mechanisms have not been fully elucidated. To identify global mRNA expression patterns that are potentially regulated by YB-1, a previously established and well-characterized cell model derived from drug-sensitive (EPG85-257P/tetR/YB-1) and multidrug-resistant (EPG85-257RDB/tetR/YB-1) gastric carcinoma cells in which the expression of YB-1 can be inhibited by tetracycline-dependent activation of the RNA interference (RNAi) pathway, was analyzed by microarray technology. By this approach, various potentially regulated genes encoding members of important cellular pathways such as the Jak/STAT, VEGF and the MAP-kinase signaling pathways were identified. Independent validation of these findings by quantitative real-time reverse transcriptase polymerase chain reaction and Western blot did not confirm these regulatory effects. In conclusion, the findings suggest that YB-1 is not directly involved in the regulation of mRNA expression in drug-sensitive or drug-resistant gastric carcinoma cells.
Y 盒结合蛋白 1（YB-1）是一种在多种哺乳动物细胞中表达的多功能细胞蛋白，包括人类癌细胞。它参与调节各种基因，包括与癌症相关的基因，但靶基因的全部范围和调节机制尚未完全阐明。为了鉴定可能受 YB-1 调节的全局 mRNA 表达模式，我们利用先前建立的、特征明确的细胞模型进行了分析，该模型源自对四环素依赖性 RNA 干扰（RNAi）通路激活敏感（EPG85-257P/tetR/YB-1）和多药耐药（EPG85-257RDB/tetR/YB-1）的胃癌细胞，YB-1 的表达可以通过该通路抑制。通过微阵列技术，鉴定了各种潜在调节基因，这些基因编码重要细胞途径的成员，如 Jak/STAT、VEGF 和 MAP 激酶信号通路。通过定量实时逆转录聚合酶链反应和 Western blot 对这些发现进行独立验证并未证实这些调节作用。总之，这些发现表明 YB-1 不直接参与调节敏感或耐药胃癌细胞中的 mRNA 表达。
