Heim Steffen, Lage Hermann
Europroteome AG, Berlin-Hennigsdorf, Berlin, Germany.
In Vivo. 2005 May-Jun;19(3):583-90.
Multidrug resistance (MDR) of human cancers is the major cause of failure of chemotherapy. To better understand the molecular events associated with the development of different types of MDR, two different multidrug-resistant gastric carcinoma cell lines, the MDR1/P-glycoprotein-expressing cell line EPG85-257RDB and the MDR1/P-glycoprotein-negative cell variant EPG85-257RNOV, as well as the corresponding drug-sensitive parental cell line EPG85-257P, were used for analyses of the mRNA expression profiles by cDNA array hybridization. Of more than 12,000 genes spotted on the arrays, 156 genes were detected as being significantly regulated in the cell line EPG85-257RDB in comparison to the non-resistant cell variant, and 61 genes were found to be differentially expressed in the cell line EPG85-257RNOV Seventeen genes showed a differential expression level in both multidrug-resistant gastric carcinoma variants. The impact of these alterations in gene expression levels in different multidrug-resistant gastric carcinoma cell variants is discussed.
人类癌症的多药耐药性（MDR）是化疗失败的主要原因。为了更好地理解与不同类型多药耐药性发展相关的分子事件，使用了两种不同的多药耐药胃癌细胞系，即表达MDR1/P-糖蛋白的细胞系EPG85-257RDB和MDR1/P-糖蛋白阴性的细胞变体EPG85-257RNOV，以及相应的药物敏感亲本细胞系EPG85-257P，通过cDNA阵列杂交分析mRNA表达谱。在阵列上点样的12000多个基因中，与非耐药细胞变体相比，在细胞系EPG85-257RDB中检测到156个基因有显著调控，在细胞系EPG85-257RNOV中发现61个基因有差异表达。17个基因在两种多药耐药胃癌变体中均显示出差异表达水平。讨论了这些基因表达水平变化在不同多药耐药胃癌细胞变体中的影响。
