Department of Bacteriology I, National Institute of Infectious Diseases, Shinjuku-ku, Tokyo, Japan.
Jpn J Infect Dis. 2010 Mar;63(2):119-23.
Ethidium monoazide (EMA) and propidium monoazide (PMA) have been utilized for selective PCR amplification of DNA from viable bacterial cells. In this study, we compared the abilities of EMA and PMA, together with real-time PCR, to specifically distinguish dead Legionella cells from viable cells. Several experiments showed that PMA or EMA treatment could specifically prevent the PCR amplification of DNA from dead Legionella cells in water samples. However, a 4-fold higher concentration of PMA than EMA was required to achieve this effect. EMA may therefore be more useful for practical environmental investigations of Legionella.
吖啶橙（EMA）和吖啶丙啶（PMA）已被用于选择性地扩增活细菌细胞的 DNA 的聚合酶链反应（PCR）。在本研究中，我们比较了 EMA 和 PMA 与实时 PCR 一起特异性区分活军团菌细胞与死菌细胞的能力。多项实验表明，PMA 或 EMA 处理可特异性阻止水样中死军团菌细胞的 DNA 进行 PCR 扩增。然而，PMA 比 EMA 达到此效果所需的浓度高 4 倍。因此，EMA 可能更适用于军团菌的实际环境研究。
