Westfalian Wilhelms University Münster, Münster, Germany.
Pathobiology. 2010;77(2):53-63. doi: 10.1159/000278292. Epub 2010 Mar 22.
In lung cancer, integrating translational data from various histologies obtained in different patients under different conditions can increase their robustness. This is a meta-analysis of cDNA array data obtained in 688 tumor patients (541 non-small cell lung cancer, 33 small cell lung cancer and 114 others) and 205 controls. 1,206 genes were found to be dysregulated in one of the 12 transcriptomics studies available. 748 results (62%) were obtained only once and might be questioned. 38% of observations could be reproduced twice or more. 346 genes were reported twice, 80 three times, 27 four and 5 five times. A common set of genes dysregulated in lung cancer was obtained, including BPA1, DUSP6, ASCL1, RNAS1 and S100P. p63 and CK 5/6 p63 are useful for differentiating adenocarcinoma and small cell lung cancer from squamous cell carcinoma. TFF-3 and MUC1 are over-expressed in adenocarcinoma. INSM1, SGNE1 and H2AFZ are typical for small cell lung cancer. Using a meta-analysis approach, it was possible to detect a robust set of genes differentially expressed in lung cancer and to determine a limited number of key genes linked to subtypes in lung cancer molecular pathology.
在肺癌中，整合来自不同患者在不同条件下获得的不同组织学的转化数据可以提高其稳健性。这是对 688 名肿瘤患者（541 名非小细胞肺癌、33 名小细胞肺癌和 114 名其他患者）和 205 名对照者的 cDNA 微阵列数据进行的荟萃分析。在可用的 12 项转录组学研究中的一项研究中，发现了 1206 个基因失调。748 个结果（62%）仅出现过一次，可能会受到质疑。38%的观察结果可以重复两次或更多次。有 346 个基因被报告了两次，80 个基因被报告了三次，27 个基因被报告了四次，5 个基因被报告了五次。获得了一组在肺癌中失调的常见基因，包括 BPA1、DUSP6、ASCL1、RNAS1 和 S100P。p63 和 CK 5/6 p63 可用于区分腺癌和小细胞肺癌与鳞状细胞癌。TFF-3 和 MUC1 在腺癌中过度表达。INSM1、SGNE1 和 H2AFZ 是小细胞肺癌的典型特征。使用荟萃分析方法，可以检测到一组在肺癌中差异表达的稳健基因，并确定与肺癌分子病理学亚型相关的少数关键基因。
