蛋白激酶 C 调控脊髓神经元的轴突生长。
Protein kinase C regulates neurite outgrowth in spinal cord neurons.
机构信息
Department of Anatomy, Third Military Medical University, Chongqing 400038, China.
出版信息
Neurosci Bull. 2010 Apr;26(2):117-25. doi: 10.1007/s12264-010-1105-y.
OBJECTIVE
The functional roles of protein kinase C (PKC) in the neurite outgrowth and nerve regeneration remain controversial. The present study was aimed to investigate the role of PKC in neurite outgrowth, by studying their regulatory effects on neurite elongation in spinal cord neurons in vitro.
METHODS
The anterior-horn neurons of spinal cord from embryonic day 14 (E14) Sprague-Dawley (SD) rats were dissociated, purified and cultured in the serum-containing medium. The ratio of membrane-PKC (mPKC) activity to cytoplasm-PKC (cPKC) activity (m/c-PKC) was studied at different time points during culture.
RESULTS
Between 3-11 d of culture, the change of m/c-PKC activity ratio and PKC-betaII expression in the neurite were both significantly correlated with neurite outgrowth (r=0.95, P< 0.01; r=0.73, P< 0.01, respectively). Moreover, PMA, an activator of PKC, induced a dramatic elevation in the m/c-PKC activity ratio, accompanied with the increase in neurite length (r=0.99, P< 0.01). In contrast, GF 109203X, an inhibitor of PKC, significantly inhibited neurite elongation, which could not be reversed by PMA.
CONCLUSION
PKC activity may be important in regulating neurite outgrowth in spinal cord neurons, and betaII isoform of PKC probably plays a major role in this process.
目的
蛋白激酶 C（PKC）在轴突生长和神经再生中的功能作用仍存在争议。本研究旨在通过研究 PKC 对体外脊髓神经元轴突伸长的调节作用，探讨 PKC 在轴突生长中的作用。
方法
分离培养胚胎 14 天（E14）Sprague-Dawley（SD）大鼠脊髓前角神经元，在含血清的培养基中培养。研究培养不同时间点膜 PKC（mPKC）活性与胞浆 PKC（cPKC）活性的比值（m/c-PKC）的变化。
结果
培养 3-11 天，m/c-PKC 活性比值和 PKC-βII 表达的变化与轴突生长均呈显著相关（r=0.95，P<0.01；r=0.73，P<0.01）。此外，PKC 的激活剂 PMA 可显著提高 m/c-PKC 活性比值，同时增加轴突长度（r=0.99，P<0.01）。相反，PKC 的抑制剂 GF 109203X 显著抑制轴突伸长，而 PMA 不能逆转这种抑制作用。
结论
PKC 活性可能在调节脊髓神经元轴突生长中起重要作用，PKC-βII 同工型可能在这一过程中起主要作用。
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