Department of Chemistry, Colorado State University, Fort Collins, CO, USA.
Anal Chem. 2010 May 1;82(9):3426-9. doi: 10.1021/ac9029086.
A microchip capillary electrophoresis (MCE) system has been developed for the determination of perchlorate in drinking water. The United States Environmental Protection Agency (USEPA) recently proposed a health advisory limit for perchlorate in drinking water of 15 parts per billion (ppb), a level requiring large, sophisticated instrumentation, such as ion chromatography coupled with mass spectrometry (IC-MS), for detection. An inexpensive, portable system is desired for routine online monitoring applications of perchlorate in drinking water. Here, we present an MCE method using contact conductivity detection for perchlorate determination. The method has several advantages, including reduced analysis times relative to IC, inherent portability, high selectivity, and minimal sample pretreatment. Resolution of perchlorate from more abundant ions was achieved using zwitterionic, sulfobetaine surfactants, N-hexadecyl-N,N-dimethyl-3-ammonio-1-propane sulfonate (HDAPS) and N-tetradecyl-N,N-dimethyl-3-ammonio-1-propane sulfonate (TDAPS). The system performance and the optimization of the separation chemistry, including the use of these surfactants to resolve perchlorate from other anions, are discussed in this work. The system is capable of detection limits of 3.4 +/- 1.8 ppb (n = 6) in standards and 5.6 +/- 1.7 ppb (n = 6) in drinking water.
已开发出一种用于测定饮用水中高氯酸盐的微芯片毛细管电泳（MCE）系统。美国环保署（USEPA）最近提出了饮用水中高氯酸盐的健康建议限值为 15 皮克/每十亿（ppb），这需要大型复杂的仪器，如与质谱（IC-MS）联用的离子色谱法，才能进行检测。需要一种廉价、便携式的系统，用于饮用水中高氯酸盐的常规在线监测应用。在此，我们提出了一种使用接触式电导检测的 MCE 方法，用于高氯酸盐的测定。该方法具有几个优点，包括与 IC 相比分析时间更短、固有便携性、高选择性和最小的样品预处理。使用两性离子、磺基甜菜碱表面活性剂 N-十六烷基-N，N-二甲基-3-氨丙基磺酸盐（HDAPS）和 N-十四烷基-N，N-二甲基-3-氨丙基磺酸盐（TDAPS）可实现高氯酸盐与更丰富离子的分离。本工作讨论了系统性能和分离化学的优化，包括使用这些表面活性剂从其他阴离子中分辨出高氯酸盐。该系统在标准品中的检测限为 3.4 +/- 1.8 ppb（n = 6），在饮用水中的检测限为 5.6 +/- 1.7 ppb（n = 6）。
