Ophthalmic Research Group, Research and Development Center, Santen Pharmaceutical Co. Ltd., 8916-16 Takayama-cho, Ikoma, Nara 630-0101, Japan.
J Ocul Pharmacol Ther. 2010 Apr;26(2):181-6. doi: 10.1089/jop.2009.0100.
The aim of this study was to evaluate and compare the effects of repeated administrations of three prostaglandin F(2alpha) analogs (tafluprost, latanoprost, and travoprost) on optic nerve head (ONH) blood flow in normal rabbits.
Male Dutch rabbits were housed under a 12-h light-dark cycle for use in this study. A quantitative index of blood flow, the squared blur rate (SBR), was determined using laser speckle flowgraphy. Saline, 0.0015% tafluprost, 0.005% latanoprost, or 0.004% travoprost (each 50 microL) was topically administered into the left eye once daily for 28 days. ONH blood flow was measured before the start at the course of treatment (baseline), and on day 14 and/or day 28 [measurements being made at 0, 30, and/or 60 min after drugs application on day 14 and/or day 28]. Heart rate was also measured at these time points.
Tafluprost, latanoprost, and travoprost each increased the ONH blood flow at all measurement points on day 14 and/or day 28. The 0 min SBR value on day 14 was greater than the baseline SBR value by 8.7% + or - 4.4% for tafluprost and by 5.8% + or - 1.7% for latanoprost. The 0 min SBR value on day 28 was greater than the baseline SBR value by 11.9% + or - 3.9% for tafluprost, by 7.2% + or - 4.3% for latanoprost, and by 6.7% + or - 3.5% for travoprost. The increasing state of the ONH blood flow continued within 60 min after a topical administration of tafluprost, latanoprost, or travoprost. Tafluprost, latanoprost, and travoprost did not change heart rate (vs. the baseline value) at any measurement points.
Repeated topical administration of any of the three prostaglandin F(2alpha) analogs increased ONH blood flow in rabbits, without changing heart rate. The increase in ONH blood flow induced by tafluprost was greater than that induced by latanoprost (P = 0.086) or travoprost (P = 0.037) at 60 min on day 28.
本研究旨在评估和比较三种前列腺素 F(2alpha)类似物（他氟前列素、拉坦前列素和曲伏前列素）重复给药对正常兔视神经头（ONH）血流的影响。
雄性荷兰兔在 12 小时光照-黑暗周期下饲养，用于本研究。使用激光散斑血流仪测定血流的定量指标，即平方模糊率（SBR）。左眼每日给予生理盐水、0.0015%他氟前列素、0.005%拉坦前列素或 0.004%曲伏前列素（各 50μL），连续 28 天。在治疗开始前（基线）和治疗过程中（第 14 天和/或第 28 天）测量 ONH 血流，并在第 14 天和/或第 28 天药物应用后 0、30 和/或 60 分钟测量[测量点]。这些时间点还测量了心率。
他氟前列素、拉坦前列素和曲伏前列素在第 14 天和/或第 28 天的所有测量点均增加了 ONH 血流。第 14 天 0 分钟的 SBR 值比基线 SBR 值增加 8.7%+/-4.4%，他氟前列素增加 5.8%+/-1.7%。第 28 天 0 分钟的 SBR 值比基线 SBR 值增加 11.9%+/-3.9%，他氟前列素增加 7.2%+/-4.3%，拉坦前列素增加 6.7%+/-3.5%。他氟前列素、拉坦前列素和曲伏前列素在给药后 60 分钟内持续增加 ONH 血流。他氟前列素、拉坦前列素和曲伏前列素在任何测量点均未改变心率（与基线值相比）。
重复局部给予三种前列腺素 F(2alpha)类似物中的任何一种均可增加兔的 ONH 血流，而不改变心率。与拉坦前列素（P=0.086）或曲伏前列素（P=0.037）相比，他氟前列素在第 28 天 60 分钟时诱导的 ONH 血流增加更大。
