Department of Microbiology, University of Mississippi Medical Center, 2500 North State Street, Jackson, MS 39216, USA.
J Ocul Pharmacol Ther. 2010 Apr;26(2):193-8. doi: 10.1089/jop.2009.0121.
To determine the effectiveness of topically applied besifloxacin, gatifloxacin, and moxifloxacin for the early treatment of experimental methicillin-resistant Staphylococcus aureus (MRSA) keratitis.
Ten hours post-MRSA infection, rabbit eyes were treated topically with 19 doses of phosphate-buffered saline (PBS), besifloxacin, gatifloxacin, or moxifloxacin. Slit-lamp examinations were performed before and after the inoculation. Corneas were harvested for bacterial quantitation and minimal inhibitory concentrations (MICs) were determined.
All 3 fluoroquinolones significantly lowered the clinical severity of the infection as compared to treatment with PBS (P < 0.05). However, the mean log(10) colony-forming unit (CFU) recovered from besifloxacin-treated corneas was significantly lower than all other treatment groups (P < 0.01). CFU recovered from corneas treated with moxifloxacin and PBS showed no significant difference (P = 0.12). Corneas treated with gatifloxacin had a significantly lower log(10) CFU recovered as compared to PBS-treated corneas (P < 0.01). The MICs for gatifloxacin and moxifloxacin were 8 microg/mL, whereas the MIC for besifloxacin was 1 microg/mL.
All 3 fluoroquinolones significantly lowered the clinical severity of the infection. Besifloxacin had an 8-fold lower MIC for MRSA than gatifloxacin and moxifloxacin, and was significantly more effective than gatifloxacin and moxifloxacin in reducing the number of MRSA in the rabbit cornea.
评估局部应用贝西沙星、加替沙星和莫西沙星治疗实验性耐甲氧西林金黄色葡萄球菌（MRSA）角膜炎的疗效。
在 MRSA 感染后 10 小时，用磷酸盐缓冲盐水（PBS）、贝西沙星、加替沙星或莫西沙星对兔眼进行 19 次局部治疗。在接种前后进行裂隙灯检查。采集角膜进行细菌定量，并测定最小抑菌浓度（MIC）。
与 PBS 治疗相比，所有 3 种氟喹诺酮类药物均显著降低了感染的临床严重程度（P < 0.05）。然而，贝西沙星治疗组角膜回收的平均 log10 菌落形成单位（CFU）明显低于其他所有治疗组（P < 0.01）。莫西沙星和 PBS 治疗组回收的 CFU 无显著差异（P = 0.12）。与 PBS 治疗组相比，加替沙星治疗组回收的 log10 CFU 明显减少（P < 0.01）。加替沙星和莫西沙星的 MIC 为 8 μg/mL，而贝西沙星的 MIC 为 1 μg/mL。
所有 3 种氟喹诺酮类药物均显著降低了感染的临床严重程度。贝西沙星对 MRSA 的 MIC 比加替沙星和莫西沙星低 8 倍，在减少兔眼角膜中 MRSA 数量方面明显优于加替沙星和莫西沙星。
