Rodrigues Rosangela, Manenti Sandra, Romao Pedro Roosevelt Torres, de Paula Ferreira Joao Leandro, Batista Joao Paulo Gervasio, Siqueira Antonio Flavio Almeida Carvalho, de Macedo Brigido Luis Fernando
Instituto Adolfo Lutz, Serviço de Virologia, Laboratorio de Retrovirus, São Paulo, SP, Brazil.
AIDS Res Hum Retroviruses. 2010 Mar;26(3):351-7. doi: 10.1089/aid.2009.0214.
Southern Brazil has the highest prevalence rate of AIDS in the country and is the only region in the Americas where HIV-1 C prevails. Metropolitan areas and harbor cities have been evaluated, but limited information is available for small towns and specific populations. We studied women attending the obstetric outpatient clinic of Criciuma, State of Santa Catarina in 2007 to evaluate the molecular epidemiology of HIV-1 among pregnant women living with HIV/AIDS. Forty-two cases had partial pol gene sequenced and additional partial gag and/or env genes from nine women. HIV subtyping was evaluated by phylogenetic methods and antiretroviral (ARV) drug resistance mutations (DRMs) at the Stanford Database. DRMs to one or more ARV class was observed in 20/42, 48% of cases, with 15/41, 37% with viral load <500 copies/ml. Subtype C at pol was identified in 33/42, 78.6% (95% CI: 64-89%), C mosaics (CB, CF) in 2, 4.8% (95% CI: 0.8-19%), F in 4, 9.5% (95% CI: 3-21%), and B in 3, 7.1% (95% CI: 1.8-18%). Discordance in concatenated gag/pol/env or intraregion mosaic was observed in 1/9, 11% of HIV-1 C genomes. The proportion of HIV-1 C in this study is the highest rate described in the Americas. Molecular surveillance in specific populations is instrumental for a better understanding of the Brazilian HIV epidemic.
巴西南部是该国艾滋病患病率最高的地区，也是美洲唯一流行HIV-1 C型的地区。大都市地区和港口城市已得到评估，但关于小镇和特定人群的信息有限。我们研究了2007年在圣卡塔琳娜州克里西乌马市产科门诊就诊的女性，以评估感染艾滋病毒/艾滋病的孕妇中HIV-1的分子流行病学。42例进行了部分pol基因测序，另外9名女性进行了部分gag和/或env基因测序。通过系统发育方法和斯坦福数据库中的抗逆转录病毒（ARV）药物耐药突变（DRM）评估HIV亚型。在20/42（48%）的病例中观察到对一种或多种ARV类别的DRM，其中15/41（37%）的病毒载量<500拷贝/毫升。在42例中的33例（78.6%，95%可信区间：64 - 89%）中鉴定出pol基因的C亚型，2例（4.8%，95%可信区间：0.8 - 19%）为C镶嵌型（CB、CF），4例（9.5%，95%可信区间：3 - 21%）为F亚型，3例（7.1%，95%可信区间：1.8 - 18%）为B亚型。在1/9（11%）的HIV-1 C基因组中观察到串联gag/pol/env或区域内镶嵌的不一致性。本研究中HIV-1 C型的比例是美洲报道的最高比例。对特定人群进行分子监测有助于更好地了解巴西的艾滋病毒疫情。
