Monno Laura, Brindicci Gaetano, Saracino Annalisa, Punzi Grazia, Cibelli Donatella, Lagioia Antonella, Angarano Gioacchino
Clinic of Infectious Diseases, University of Bari, Bari, Italy.
AIDS Res Hum Retroviruses. 2010 Mar;26(3):365-72. doi: 10.1089/aid.2009.0224.
Viral isolation and V3 sequencing were performed for 52 patients with non-subtype B viruses. The HIV-1 isolation rate was 93%, and 98% of isolates were characterized as NSI. V3 sequences corresponding to NSI isolates were compared to non-subtype B sequences with corresponding SI isolates from the Los Alamos database. The two sequence groups significantly differed in number of sequences with 35 amino acids, net charge, Brigg's coefficient, loss of NGS at positions 6-8, and 11/25 genotype (p < 0.0001). Substantial discrepancies in V3 variability were also observed. Basic amino acids at positions 8, 21, 23, and 24 were more frequent in SI sequences as were uncharged amino acids at positions 5, 6, 7, 8, 12, 13, 25, and 34. When characterizing paired viral isolates and V3 sequences from patients with non-subtype B HIV-1, current V3 sequence-based criteria from subtype B appeared to discriminate well between NSI and SI sequences from non-subtype B patients.
对52例感染非B亚型病毒的患者进行了病毒分离和V3测序。HIV-1分离率为93%，98%的分离株被鉴定为非SI型。将与非SI型分离株对应的V3序列与洛斯阿拉莫斯数据库中来自相应SI型分离株的非B亚型序列进行比较。两组序列在35个氨基酸的序列数量、净电荷、布里格系数、第6至8位的NGS缺失以及11/25基因型方面存在显著差异（p<0.0001）。还观察到V3变异性存在实质性差异。SI序列中第8、21、23和24位的碱性氨基酸以及第5、6、7、8、12、13、25和34位的不带电荷氨基酸更为常见。在对感染非B亚型HIV-1患者的配对病毒分离株和V3序列进行特征分析时，目前基于B亚型V3序列的标准似乎能够很好地区分非B亚型患者的非SI型和SI型序列。
