Wang Yaoli, Bai Chunxue, Wang Guansong, Wang Diane, Cheng Xiaoming, Huang Jian, Jiang Dongpo, Qian Guisheng, Wang Xiangdong
Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University, Shanghai, China.
Genet Vaccines Ther. 2010 Mar 24;8:2. doi: 10.1186/1479-0556-8-2.
Allergen-induced imbalance of specific T regulatory (Treg) cells and T helper 2 cells plays a decisive role in the development of immune response against allergens.
To evaluate effects and potential mechanisms of DNA vaccine containing ovalbumin (OVA) and Fc fusion on allergic airway inflammation.
Bronchoalveolar lavage (BAL) levels of inflammatory mediators and leukocyte infiltration, expression of CD11c+CD80+ and CD11c+CD86+ co-stimulatory molecules in spleen dendritic cells (DCs), circulating CD4+ and CD8+ T cells, Foxp3+ in spleen CD4+ T cells and spleen CD4+ T cells were measured in OVA-sensitized and challenged animals pretreated with pcDNA, OVA-pcDNA, Fc-pcDNA, and OVA-Fc-pcDNA.
OVA-Sensitized and challenged mice developed airway inflammation and Th2 responses, and decreased the proliferation of peripheral CD4+and CD8+ T cells and the number of spleen Foxp3+ Treg. Those changes with increased INF-gamma production and reduced OVA-specific IgE production were protected by the pretreatment with OVA-Fc-pcDNA.
DNA vaccine encoding both Fc and OVA showed more effective than DNA vaccine encoding Fc or OVA alone, through the balance of DCs and Treg.
变应原诱导的特异性调节性T（Treg）细胞和辅助性T2细胞失衡在针对变应原的免疫反应发展中起决定性作用。
评估含卵清蛋白（OVA）和Fc融合蛋白的DNA疫苗对变应性气道炎症的影响及潜在机制。
在经pcDNA、OVA-pcDNA、Fc-pcDNA和OVA-Fc-pcDNA预处理的OVA致敏和激发动物中，检测支气管肺泡灌洗（BAL）中炎症介质水平和白细胞浸润情况，以及脾树突状细胞（DC）中CD11c+CD80+和CD11c+CD86+共刺激分子的表达、循环CD4+和CD8+T细胞、脾CD4+T细胞中的Foxp3+以及脾CD4+T细胞。
OVA致敏和激发的小鼠出现气道炎症和Th2反应，外周CD4+和CD8+T细胞增殖减少，脾Foxp3+Treg数量减少。OVA-Fc-pcDNA预处理可保护这些变化，同时增加INF-γ产生并降低OVA特异性IgE产生。
通过DC和Treg的平衡，编码Fc和OVA的DNA疫苗比单独编码Fc或OVA的DNA疫苗更有效。
