Philippine Carabao Center National Headquarters and Genepool, Science City of Munoz, Nueva Ecija, Philippines.
Comp Immunol Microbiol Infect Dis. 2011 Jan;34(1):55-63. doi: 10.1016/j.cimid.2010.02.002. Epub 2010 Mar 23.
Characterization of CTLA-4, PD-1 and PDL-1 genes from swamp and riverine type water buffaloes was done by molecular cloning, sequencing and phylogenetic analysis. The cloned cDNA of CTLA-4, PD-1 and PDL-1 contained an open reading frame of 666, 849 and 870 nucleotides, encoding a polypeptide of 221, 282 and 298 amino acids, respectively. Nucleotide sequence homology of both CTLA-4 and PDL-1 had 99.8% in swamp and riverine type, which gives the identical polypeptide. Meanwhile, PD-1 genes of swamp and riverine type water buffaloes had 99.2% of homology in nucleotide sequence, which has substitution of two amino acid residues. The hexapeptide motif, phosphatidylinositol 3'-kinase and potential glycosylation sites were conserved within the tribe Bovinae. Phylogenetic analysis confirmed the degree of relationship between the bubaline species and justify the distinctness of each breeds by the bootstrap value generated.
通过分子克隆、测序和系统发育分析，对沼泽型和河流型水牛的 CTLA-4、PD-1 和 PDL-1 基因进行了特征描述。克隆的 CTLA-4、PD-1 和 PDL-1 cDNA 含有 666、849 和 870 个核苷酸的开放阅读框，分别编码 221、282 和 298 个氨基酸的多肽。沼泽型和河流型水牛的 CTLA-4 和 PDL-1 的核苷酸序列同源性均为 99.8%，这使得它们具有相同的多肽。同时，沼泽型和河流型水牛的 PD-1 基因在核苷酸序列上有 99.2%的同源性，其中有两个氨基酸残基的替换。六肽基序、磷酸肌醇 3'-激酶和潜在的糖基化位点在牛科内是保守的。系统发育分析证实了水牛种间的亲缘关系程度，并通过生成的自举值证明了每个品种的独特性。
