Khan A A, Yong S, Prior M G, Lillie L E
Animal Sciences Division, Alberta Environmental Centre, Vegreville, Canada.
J Toxicol Environ Health. 1991 May;33(1):57-64. doi: 10.1080/15287399109531505.
Respiratory rates (basal and zymosan-stimulated) and cell viability were monitored in pulmonary alveolar macrophages (PAM) from rats exposed to 0, 70, 280, and 560 mg/m3 (0, 50, 200, and 400 ppm) hydrogen sulfide (H2S) gas for 4 h. Zymosan-stimulated respiratory rates were markedly reduced in PAM collected from rats exposed to 280 and 560 mg/m3 H2S; however, their basal respiratory rates were not affected. Significant decrease in cell viability was also observed in samples from 560 mg/m3 H2S-treated rats, but it remained high and unchanged in other treatments. In vitro incubation of PAM from control rats with sulfide (a precursor of H2S) and its two oxidation products, sulfite and sulfate, showed that sulfide was markedly more inhibitory to both respiratory rates than sulfite or sulfate. These treatments did not affect cell viability.
监测暴露于0、70、280和560毫克/立方米（0、50、200和400 ppm）硫化氢（H₂S）气体4小时的大鼠肺泡巨噬细胞（PAM）的呼吸频率（基础和酵母聚糖刺激的）和细胞活力。从暴露于280和560毫克/立方米H₂S的大鼠收集的PAM中，酵母聚糖刺激的呼吸频率显著降低；然而，它们的基础呼吸频率未受影响。在560毫克/立方米H₂S处理的大鼠样本中也观察到细胞活力显著下降，但在其他处理中细胞活力保持高且不变。用硫化物（H₂S的前体）及其两种氧化产物亚硫酸盐和硫酸盐对对照大鼠的PAM进行体外孵育，结果表明，硫化物对两种呼吸频率的抑制作用明显大于亚硫酸盐或硫酸盐。这些处理不影响细胞活力。
