Rucker Joseph, Davidoff Candice, Doranz Benjamin J
Integral Molecular, Inc, Philadelphia, PA, USA.
Methods Mol Biol. 2010;617:445-56. doi: 10.1007/978-1-60327-323-7_32.
Membrane proteins, such as G protein-coupled receptors (GPCRs) and ion channels, represent important but technically challenging targets for the management of pain and other diseases. Studying their interactions has enabled the development of new therapeutics, diagnostics, and research reagents, but biophysical manipulation of membrane proteins is often difficult because of the requirement of most membrane proteins for an intact lipid bilayer. Here, we describe the use of virus-like particles as presentation vehicles for cellular membrane proteins ("Lipoparticles"). The methods for using Lipoparticles on optical biosensors, such as the BioRad ProteOn XPR36, are discussed as a means to characterize the kinetics, affinity, and specificity of antibody interactions using surface plasmon resonance detection.
膜蛋白，如G蛋白偶联受体（GPCRs）和离子通道，是治疗疼痛和其他疾病的重要靶点，但技术上具有挑战性。对它们相互作用的研究推动了新型治疗药物、诊断方法和研究试剂的开发，然而，由于大多数膜蛋白需要完整的脂质双层环境，对其进行生物物理操作往往很困难。在此，我们描述了使用病毒样颗粒作为细胞膜蛋白的展示载体（“脂质颗粒”）。还讨论了在光学生物传感器（如伯乐公司的ProteOn XPR36）上使用脂质颗粒的方法，以此作为利用表面等离子体共振检测来表征抗体相互作用的动力学、亲和力和特异性的手段。
