Reisdorph Nichole A, Reisdorph Richard
Department of Immunology, National Jewish Health, University of Colorado, Denver, CO, USA.
Methods Mol Biol. 2010;617:457-73. doi: 10.1007/978-1-60327-323-7_33.
Technological innovations have increased our potential to evaluate global changes in protein and small molecule levels in a rapid and comprehensive manner. This is especially true in mass spectrometry-based research where improvements, including ease-of-use, in high performance liquid chromatography (HPLC), column chemistries, instruments, software, and molecular databases have advanced the fields of proteomics and metabolomics considerably. Applications of these technologies in clinical research include biomarker discovery, drug targeting, and elucidating molecular networks, and a systems-based approach, utilizing multiple "omics," can also be taken. While the exact choice of workflow can dramatically impact the results of a study, the basic steps are similar, both within and between metabolomics and proteomics experiments. Although gel-based methods of quantitation are still widely used, our laboratory focuses on mass spectrometry-based methods, specifically protein and small molecule profiling.
技术创新提高了我们以快速、全面的方式评估蛋白质和小分子水平全球变化的潜力。在基于质谱的研究中尤其如此，其中包括高效液相色谱（HPLC）、柱化学、仪器、软件和分子数据库等方面的改进，包括易用性方面的改进，极大地推动了蛋白质组学和代谢组学领域的发展。这些技术在临床研究中的应用包括生物标志物发现、药物靶向和阐明分子网络，也可以采用基于系统的方法，利用多种“组学”技术。虽然工作流程的具体选择会极大地影响研究结果，但代谢组学和蛋白质组学实验内部以及之间的基本步骤是相似的。尽管基于凝胶的定量方法仍被广泛使用，但我们实验室专注于基于质谱的方法，特别是蛋白质和小分子分析。
