Department of Histology & Embryology, The Third Military Medical University, Chongqing, 400038, China.
Cell Mol Neurobiol. 2010 Aug;30(6):869-76. doi: 10.1007/s10571-010-9515-y. Epub 2010 Mar 25.
The aim of this study was to screen genes related to the development and injury of the mouse optic nerve so as to provide possible target genes for gene-engineering therapy of central nervous system (CNS) injury. Gene expression was profiled by cDNA microarrays in the mouse superior colliculus at 8-time points during the development or following injury of the optic nerve; consequently, 1,095 highly expressed genes (ratio > or =2) were identified. Then, these genes were categorized functionally; there were 561 genes (51.19%) with unidentified functions and 534 genes (48.81%) with identified or partially identified functions. After discounting the overlapping genes, 486 genes with identified or partially identified functions were categorized into 17 functional groups. The 17 functional groups were as follows: I transcription regulation, II signal transduction, III protein synthesis, IV materials transporting, V RNA processing, VI metabolism-related genes, VII cell cycle or apoptosis-related genes, VIII extracellular matrix, IX protein folding and degradation, X cytoskeleton, XI histone metabolism, XII nervous system specific functional genes, XIII tumor related genes, XIV DNA replication and repair, XV axon growth and guidance, XVI immune response, and XVII cell adhesion. These genes may play key roles in the development, injury, and repairment of the optic nerve.
本研究旨在筛选与小鼠视神经发育和损伤相关的基因，以期为中枢神经系统（CNS）损伤的基因工程治疗提供可能的靶基因。通过在小鼠顶盖的 8 个时间点对 cDNA 微阵列进行基因表达谱分析，发现了 1095 个高度表达的基因（比率≥2）；随后对这些基因进行了功能分类；其中 561 个基因（51.19%）具有未知功能，534 个基因（48.81%）具有已知或部分已知功能。在扣除重叠基因后，有 486 个具有已知或部分已知功能的基因被归类为 17 个功能组。这 17 个功能组如下：I 转录调控，II 信号转导，III 蛋白质合成，IV 物质转运，V RNA 加工，VI 代谢相关基因，VII 细胞周期或凋亡相关基因，VIII 细胞外基质，IX 蛋白质折叠和降解，X 细胞骨架，XI 组蛋白代谢，XII 神经系统特异功能基因，XIII 肿瘤相关基因，XIV DNA 复制和修复，XV 轴突生长和导向，XVI 免疫反应，以及 XVII 细胞黏附。这些基因可能在视神经的发育、损伤和修复中发挥关键作用。
