Chenoweth Josh G, Tesar Paul J
Laboratory of Molecular Biology, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD, USA.
Methods Mol Biol. 2010;636:25-44. doi: 10.1007/978-1-60761-691-7_2.
Epiblast stem cells (EpiSCs) are isolated from the postimplantation mouse embryo just after implantation but prior to gastrulation. EpiSCs are pluripotent and provide a tractable, in vitro system to study the processes that function during gastrulation to transition pluripotent cells to their differentiated derivatives. This chapter describes the methods for the isolation and maintenance of mouse EpiSCs. We also describe basic assays used to characterize new EpiSC lines.
上胚层干细胞（EpiSCs）是在小鼠胚胎着床后、原肠胚形成之前从着床后的胚胎中分离出来的。EpiSCs具有多能性，为研究原肠胚形成过程中使多能细胞转变为其分化衍生物的机制提供了一个易于处理的体外系统。本章描述了小鼠EpiSCs的分离和培养方法。我们还描述了用于鉴定新的EpiSC系的基本检测方法。
Zotero 插件