用表观遗传抑制剂重编程小鼠上胚层干细胞。
Reprogram Murine Epiblast Stem Cells by Epigenetic Inhibitors.
作者信息
Zhang Hui, Dou Yali
机构信息
Department of Pathology, University of Michigan, Ann Arbor, USA.
出版信息
Bio Protoc. 2017 Mar 5;7(5). doi: 10.21769/BioProtoc.2168.
Abstract
Pluripotent stem cells in the naïve state are highly useful in regenerative medicine and tissue engineering. A robust reprogramming of the primed murine Epiblast Stem Cells (EpiSCs) to naïve pluripotency is feasible via chemical-only approach. This protocol described a method to reprogram murine EpiSCs by MM-401 treatment, which blocks histone H3K4 methylation by MLL1/KMT2A.
摘要
处于原始状态的多能干细胞在再生医学和组织工程中非常有用。通过仅使用化学方法，将已分化的小鼠上胚层干细胞（EpiSCs）强有力地重编程为原始多能性是可行的。本方案描述了一种通过MM-401处理对小鼠EpiSCs进行重编程的方法，MM-401可阻断MLL1/KMT2A介导的组蛋白H3K4甲基化。
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本文引用的文献
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MLL1 Inhibition Reprograms Epiblast Stem Cells to Naive Pluripotency.MLL1抑制将上胚层干细胞重编程为原始多能性。
Cell Stem Cell. 2016 Apr 7;18(4):481-94. doi: 10.1016/j.stem.2016.02.004. Epub 2016 Mar 17.
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