通过重编程和化学方法生成新型大鼠和人类多能干细胞。
Generation of novel rat and human pluripotent stem cells by reprogramming and chemical approaches.
作者信息
Li Wenlin, Ding Sheng
机构信息
Department of Chemistry, The Scripps Research Institute, La Jolla, CA, USA.
出版信息
Methods Mol Biol. 2010;636:293-300. doi: 10.1007/978-1-60761-691-7_18.
Although embryonic stem cells (ESCs) have been established from mice since 1981, attempts to derive its counterparts from various other mammals, including rats, have not succeeded. Recently, induced pluripotent stem cells (iPSCs) have been generated from both mouse and human somatic cells by genetic transduction. We had successfully established novel rat iPSCs (riPSCs), which can be homogenously maintained by LIF and a cocktail of ALK5 inhibitor, GSK3 inhibitor and MEK inhibitor. riPSCs share conventional mouse ESC characteristics and most importantly can contribute extensively to chimeras. We also generated novel human iPSCs (hiPSCs) with "mouse ESC-like" characteristics, which can be surprisingly maintained in culture in the presence of MEK inhibitor and ALK5 inhibitor.
尽管自1981年起就已从小鼠中建立了胚胎干细胞（ESC），但尝试从包括大鼠在内的各种其他哺乳动物中获得其对应细胞却未成功。最近，通过基因转导从小鼠和人类体细胞中产生了诱导多能干细胞（iPSC）。我们成功建立了新型大鼠iPSC（riPSC），其可通过白血病抑制因子（LIF）以及ALK5抑制剂、糖原合成酶激酶3（GSK3）抑制剂和丝裂原活化蛋白激酶激酶（MEK）抑制剂的混合物进行均匀维持。riPSC具有传统小鼠ESC的特征，最重要的是能够广泛地参与嵌合体的形成。我们还产生了具有“小鼠ESC样”特征的新型人类iPSC（hiPSC），令人惊讶的是，其在MEK抑制剂和ALK5抑制剂存在的情况下能够在培养中维持。
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