Chen Jingchen, Morosan-Puopolo Gabriela, Dai Fangping, Wang Jianlin, Brand-Saberi Beate
Department of Molecular Embryology, Institute of Anatomy and Cell Biology, University of Freiburg, Germany.
Int J Dev Biol. 2010;54(5):925-9. doi: 10.1387/ijdb.092933jc.
Cecr2 is a transcription factor involved in neurulation and chromatin remodeling. In the present study, the full length of the coding sequence of the chicken orthologue Cecr2 was obtained by RT-PCR. Sequence analysis and alignment showed that it contained an AT hook, as well as a bromodomain which was highly conserved among different species, consistent with its role in chromatin remodeling. The expression pattern of chicken Cecr2 was subsequently investigated during the development of the chicken embryo by in situ hybridization. In addition to its predominant expression in neural tissues during neurulation, Cecr2 was also found to be expressed in the developing somites and in the intermediate zone of the spinal cord, suggesting that it may play a role in somite and neuronal development.
Cecr2是一种参与神经胚形成和染色质重塑的转录因子。在本研究中，通过逆转录聚合酶链反应（RT-PCR）获得了鸡同源基因Cecr2编码序列的全长。序列分析和比对表明，它含有一个AT钩以及一个在不同物种间高度保守的溴结构域，这与其在染色质重塑中的作用相一致。随后通过原位杂交研究了鸡Cecr2在鸡胚胎发育过程中的表达模式。除了在神经胚形成期间在神经组织中占主导地位的表达外，还发现Cecr2在发育中的体节和脊髓中间区表达，这表明它可能在体节和神经元发育中发挥作用。
