Li Xiaowei, Yang Hao, Wan Lin, Lu Xiaofeng
Key Lab of Transplant Engineering and Immunology, West China Hospital, Sichuan University, Chengdu 610041, China.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2010 Feb;27(1):142-6.
Broad-spectrum of conventional antibiotics is one of the key factors that cause antibiotics-resistance of many bacteria. Bacteriocins are regarded as the next generation of antibiotics on account of their narrow-spectrum bactericidal activities. Many attentions have been paid to colicins because they are believed to be safe in regard to human body. In this paper, the genes encoding colicin S4 and its immunity protein were cloned into pQE30 to produce colicin S4 expression vector pQE30-Col S4. Colicin S4 was highly expressed as soluble form in gE colig M15 containing pQE30-Col S4. The yields ranged from 30 mg/L to 50 mg/L. The recombinant colicin S4 with an additional 6 His-tag at its N-terminus was found being similar to the natural colicin S4 in antibacterial activity. It only showed bactericidal activity against E. coli strains, thus makig it attractive to develop this protein as a novel antibiotic with narrow spectrum.
广谱常规抗生素是导致许多细菌产生抗生素耐药性的关键因素之一。细菌素因其窄谱杀菌活性而被视为下一代抗生素。由于人们认为大肠杆菌素对人体安全，因此受到了广泛关注。在本文中，将编码大肠杆菌素S4及其免疫蛋白的基因克隆到pQE30中，构建大肠杆菌素S4表达载体pQE30-Col S4。在含有pQE30-Col S4的大肠杆菌M15中，大肠杆菌素S4以可溶性形式高效表达。产量范围为30 mg/L至50 mg/L。发现其N端带有额外6个组氨酸标签的重组大肠杆菌素S4在抗菌活性方面与天然大肠杆菌素S4相似。它仅对大肠杆菌菌株表现出杀菌活性，因此将这种蛋白质开发为一种新型窄谱抗生素具有吸引力。
