Genomics Research Center, Academia Sinica Taipei 115, Taiwan, R.O.C.
Biomacromolecules. 2010 Apr 12;11(4):994-1001. doi: 10.1021/bm901461e.
Recent progress has led to the identification of liver stem/progenitor cells as suitable sources for generating transplantable liver cells. However, the great variability in methods utilized to isolate liver stem/progenitor cells is a considerable challenge for clinical applications. The polyelectrolyte-multilayer technique can constitute a useful method for selective cell adhesion. Whether enrichment of liver stem/progenitor cells can be achieved utilizing polypeptide polyelectrolyte-multilayer films was investigated in current work. Fetal liver cells isolated from E13.5 mouse embryos were seeded on the poly-l-glutamic acid/poly-l-lysine alternating films, and we revealed that fetal liver stem/progenitor cells were selected and formed colonies. These undifferentiated colonies were maintained on the films composed of four alternating layers, with the topmost poly-l-glutamic acid layer judged by the constitutive expression of stem-cell markers such as Dlk-1, CD49f, and CD133 and self-renew marker-beta-catenin. Our work has demonstrated that highly tunable polyelectrolyte-multilayer films were suitable for selective enrichment of liver stem/progenitor cells in vitro.
最近的研究进展使得肝干细胞/祖细胞成为生成可移植肝细胞的合适来源。然而，用于分离肝干细胞/祖细胞的方法存在很大差异，这对临床应用来说是一个巨大的挑战。聚电解质多层技术可以构成一种用于选择性细胞黏附的有用方法。本研究旨在探讨利用多肽聚电解质多层膜能否实现肝干细胞/祖细胞的富集。将从 E13.5 胎鼠肝脏中分离得到的肝母细胞接种到聚谷氨酸/聚赖氨酸交替膜上，结果表明，胎肝干细胞/祖细胞被选择性地黏附并形成集落。这些未分化的集落可在由四层交替层组成的膜上维持生长，最顶层的聚谷氨酸层可通过干细胞标志物如 Dlk-1、CD49f 和 CD133 的组成型表达以及自我更新标志物β-连环蛋白来判断。我们的工作表明，高度可调的聚电解质多层膜适合于体外选择性富集肝干细胞/祖细胞。
