Laboratório de Química de Produtos Naturais, Centro de Pesquisas René Rachou, Belo Horizonte, Brazil.
Mycoses. 2011 Jul;54(4):e122-9. doi: 10.1111/j.1439-0507.2009.01854.x. Epub 2010 Mar 11.
Paracoccidioidomycosis (PCM), a human mycosis caused by the dimorphic fungus Paracoccidioides brasiliensis, is a serious public health problem in several countries of Latin America. In our search we found that the crude extract of the endophytic fungus UFMGCB 551 was able to inhibit several clinical strains of P. brasiliensis, and was also active in the bioautographic assay against Cladosporium sphaerospermum. The endophytic fungus UFMGCB 551 was isolated from the plant Piptadenia adiantoides J.F. Macbr (Fabaceae). The fungus was identified as Fusarium sp. based on its macro- and micro-morphology, and on the sequence of the internally transcribed spacer regions (ITS) of its rRNA gene. The chromatographic fractionation of the fungal extract was guided by the bioautographic assay to afford three known trichothecene mycotoxins: T2-toxin (1) and a mixture of 8-n-butyrylneosolaniol (2) and 8-isobutyrylsolaniol (3). The minimal inhibitory concentrations (MIC) of the these compounds against eleven clinical strains of P. brasiliensis were evaluated and found to be in the range between 75 and 640 nmol l(-1) for 1 and 160-640 nmol l(-1) for the mixture of 2 and 3.
巴西副球孢子菌病（PCM）是一种由双相真菌巴西副球孢子菌引起的人类真菌病，是拉丁美洲几个国家的严重公共卫生问题。在我们的研究中，我们发现内生真菌 UFMGCB 551 的粗提物能够抑制几种临床分离株的巴西副球孢子菌，并且在针对枝孢属 Cladosporium sphaerospermum 的生物自显影测定中也具有活性。内生真菌 UFMGCB 551 是从植物 Piptadenia adiantoides J.F. Macbr（豆科）中分离出来的。该真菌基于其宏观和微观形态，以及其 rRNA 基因内转录间隔区（ITS）的序列，被鉴定为镰刀菌属。真菌提取物的色谱分离受生物自显影测定指导，得到三种已知的三萜类霉菌毒素：T2 毒素（1）和 8-正丁酰-neosolaniol（2）和 8-异丁酰-solaniol（3）的混合物。这些化合物对 11 株临床分离株巴西副球孢子菌的最小抑菌浓度（MIC）进行了评估，发现其范围为 75-640 nmol l（-1），对于 2 和 3 的混合物为 160-640 nmol l（-1）。
