Department of Applied Chemistry, National Chiao Tung University, Hsinchu 300, Taiwan.
J Chromatogr A. 2010 Jun 25;1217(26):4471-5. doi: 10.1016/j.chroma.2010.02.085. Epub 2010 Mar 6.
In this study, nonaqueous capillary electrophoresis (NACE) was used to separate three open-cage fullerenes. Trifluoroacetic acid (TFA) was used as the nonaqueous background electrolyte to change the analytes' mobilities. The selectivity and separation efficiency were critically affected by the nature of the buffer system, the choice of organic solvent, and the concentrations of TFA and sodium acetate (NaOAc) in the background electrolyte. The optimized separation occurred using 200 mM TFA/20mM NaOAc in MeOH/acetonitrile (10:90, v/v), providing highly efficient baseline separation of the open-cage fullerenes within 5 min. The migration time repeatability for the three analytes was less than 1% (relative standard deviation). Thus, NACE is a rapid, useful alternative to high-performance liquid chromatography for the separation of open-cage fullerenes.
在这项研究中，使用非水毛细管电泳（NACE）分离了三种开笼富勒烯。三氟乙酸（TFA）用作非水背景电解质，以改变分析物的迁移率。缓冲体系的性质、有机溶剂的选择以及背景电解质中 TFA 和醋酸钠（NaOAc）的浓度对选择性和分离效率有重要影响。在 MeOH/乙腈（10:90，v/v）中使用 200 mM TFA/20 mM NaOAc 作为优化分离条件，可以在 5 分钟内实现开笼富勒烯的高效基线分离。三种分析物的迁移时间重复性小于 1%（相对标准偏差）。因此，与高效液相色谱相比，NACE 是一种快速、有用的分离开笼富勒烯的替代方法。
