Department of Pharmacology, School of Chemical Biology & Pharmaceutical Sciences, Capital Medical University, Beijing 100069, PR China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2010 Apr 15;878(13-14):915-24. doi: 10.1016/j.jchromb.2010.02.014. Epub 2010 Feb 18.
Tanshinone I and its analogue dihydrotanshinone I are the major active components isolated from Salvia miltiorrhiza Bunge and Salvia Przewalskii Maxim. These compounds have been found to possess significant antibacterial, anti-dermatophytic, antioxidant, anti-inflammatory and anticancer activities. Fifteen phase I metabolites and two phase II metabolites of tanshinone I and dihydrotanshinone I in rat bile were elucidated and identified by a sensitive HPLC-ESI-MS(n) method. The molecular structures of the metabolites are presented on the basis of the characteristics of their precursor ions, product ions and chromatographic retention times. The results indicate that the phase I metabolites are biotransformed through four main pathways: dehydrogenation, hydroxylation, furan ring cleavage and oxidation metabolism. Phase II metabolites were mainly identified as the sulfated conjugates which showed a characteristic neutral loss of 80 Da. The biotransformed pathways of tanshinone I and dihydrotanshinone I were proposed on the basis of the investigation.
丹参酮 I 及其类似物二氢丹参酮 I 是从丹参和紫丹参中分离得到的主要活性成分。这些化合物已被发现具有显著的抗菌、抗真菌、抗氧化、抗炎和抗癌活性。采用灵敏的 HPLC-ESI-MS(n)法阐明并鉴定了大鼠胆汁中丹参酮 I 和二氢丹参酮 I 的 15 种 I 相代谢物和 2 种 II 相代谢物。根据前体离子、产物离子和色谱保留时间的特征，提出了代谢物的分子结构。结果表明，I 相代谢物通过脱氢、羟化、呋喃环裂解和氧化代谢四种主要途径进行生物转化。II 相代谢物主要鉴定为硫酸结合物，其特征性中性丢失 80 Da。基于研究结果提出了丹参酮 I 和二氢丹参酮 I 的生物转化途径。
