Castro Luciano A, Elias Larissa S A, Oton-Leite Angélica F, de Spíndula-Filho José V, Leles Claudio R, Batista Aline C, Mendonça Elismauro F
Department of Oral Medicine (Oral Pathology), Dental School, Federal University of Goiás, Goiânia, Brazil.
J Oral Sci. 2010 Mar;52(1):55-62. doi: 10.2334/josnusd.52.55.
The chronic usage of nifedipine is associated with the appearance of gingival overgrowth (GO). The frequency of GO associated with chronic nifedipine therapy remains controversial and the possible subclinical effects of this drug on the gingival epithelium should be investigated. We investigated the epithelial proliferation index and apoptosis rate, and their association with epithelial enlargement. Proliferation (Ki67 and Cyclin B1) and apoptosis (BCL2, Bax and p53) markers were identified by immunohistochemistry in twenty-one samples of gingival tissue from patients undergoing chronic treatment with nifedipine and in eleven samples of gingival tissue from healthy patients who did not use drugs associated with GO (control). Our results show that the epithelial tissue of nifedipine users has considerably longer rete pegs compared to control (P = 0.01). However, the density of Ki67(+) and Cyclin B1(+) cells was similar in both groups. Regarding apoptosis, we found more BCL2(+) cells in the nifedipine group when compared to controls (P = 0.12). An increase in Bax(+) cells in the nifedipine group compared to control (P = 0.003) was also seen, and slightly lower levels of p53(+) expression were observed (P = 0.51). Our results suggest that the chronic use of nifedipine is not associated with subclinical changes in gingival tissue.
硝苯地平的长期使用与牙龈增生（GO）的出现有关。与慢性硝苯地平治疗相关的GO发生率仍存在争议，该药物对牙龈上皮可能的亚临床影响值得研究。我们研究了上皮增殖指数和凋亡率，以及它们与上皮增大的关系。通过免疫组织化学在21例接受硝苯地平慢性治疗患者的牙龈组织样本和11例未使用与GO相关药物的健康患者（对照）的牙龈组织样本中鉴定增殖（Ki67和细胞周期蛋白B1）和凋亡（BCL2、Bax和p53）标志物。我们的结果表明，与对照组相比，硝苯地平使用者的上皮组织的 rete 嵴长得多（P = 0.01）。然而，两组中Ki67(+)和细胞周期蛋白B1(+)细胞的密度相似。关于凋亡，与对照组相比，硝苯地平组中BCL2(+)细胞更多（P = 0.12）。与对照组相比，硝苯地平组中Bax(+)细胞也有所增加（P = 0.003），并且观察到p53(+)表达水平略低（P = 0.51）。我们的结果表明，硝苯地平的长期使用与牙龈组织的亚临床变化无关。
