Department of Clinical Medicine, Chongqing Medical and Pharmaceutical College, Chongqing, China.
Horm Metab Res. 2010 Jun;42(7):521-7. doi: 10.1055/s-0030-1249019. Epub 2010 Mar 25.
Melanin-concentrating hormone receptor 2 (MCHR2), a second G protein-coupled receptor for melanin-concentrating hormone (MCH), has been known for many years. However, its physiological function is poorly understood. To identify the proteins involved in MCHR2 physiological function, a comparative proteomic analysis of protein expression in SH-SY5Y cells stably expressing human MCHR2 (SH-SY5Y-MCHR2) and control SH-SY5Y cells (SH-SY5Y-mock) - both treated with MCH - was conducted. Significant changes were observed in the expression of 34 proteins, including isocitrate dehydrogenase (NAD) subunit alpha, mitochondrial (IDH3A), phosphoenolpyruvate carboxykinase 1 (PCK1), 6-phosphofructo-2-kinase/fructose-2.6-biphosphatase 4 (PFKFB4), insulin-induced gene 2 protein (INSIG2), and acyl-coenzyme A thioesterase 8 (ACOT8). Among the proteins, IDH3A, PCK1, PFKFB4 increased significantly, and INSIG2, ACOT8 decreased significantly in experimental cells compared with control cells; these findings were further confirmed by semi-quantitative RT-PCR and Western blot analysis. The comparative proteome data may provide a valuable clue to further understand MCHR2 physiological function, and several differentially regulated proteins may be used as target proteins for the development of novel drugs.
黑色素浓缩激素受体 2（MCHR2）是黑色素浓缩激素（MCH）的第二种 G 蛋白偶联受体，多年来一直为人所知。然而，其生理功能尚不清楚。为了鉴定参与 MCHR2 生理功能的蛋白质，对稳定表达人 MCHR2（SH-SY5Y-MCHR2）和对照 SH-SY5Y 细胞（SH-SY5Y-mock）的 SH-SY5Y 细胞中蛋白质表达的比较蛋白质组学分析进行了研究 - 两者均用 MCH 处理。观察到 34 种蛋白质的表达发生了显着变化，包括异柠檬酸脱氢酶（NAD）亚基α，线粒体（IDH3A），磷酸烯醇丙酮酸羧激酶 1（PCK1），6-磷酸果糖-2-激酶/果糖-2.6-二磷酸酶 4（PFKFB4），胰岛素诱导基因 2 蛋白（INSIG2）和酰基辅酶 A 硫酯酶 8（ACOT8）。在实验细胞中，与对照细胞相比，IDH3A、PCK1、PFKFB4 显着增加，INSIG2、ACOT8 显着减少；这些发现通过半定量 RT-PCR 和 Western blot 分析得到进一步证实。比较蛋白质组数据可能为进一步了解 MCHR2 的生理功能提供有价值的线索，并且几种差异调节的蛋白质可用作开发新型药物的靶标蛋白。
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