Vascular Biology Center, Medical College of Georgia, Augusta, GA 30912, USA.
Int J Immunogenet. 2010 Jun;37(3):205-12. doi: 10.1111/j.1744-313X.2010.00910.x. Epub 2010 Mar 14.
To identify HLA-B15 subtypes distribution in Han population in Beijing, People's Republic of China, 826 unrelated healthy individuals were typed using the polymerase chain reaction-sequence-based typing method. Within the 246 HLA-B15 positive individuals, 29 HLA-B15 alleles were identified, the most predominant of which is B1501 (40.07%), followed by B1502 (12.87%), B1511 (12.87%), B1518 (9.19%) and B1532 (3.31%). The distribution of HLA-B*15 subtype frequencies was compared between the Beijing Han, eight other Chinese ethnic minorities and six Chinese populations covering the mainland of China, Taiwan, Hong Kong and Singapore. A neighbor-joining phylogenetic tree was constructed and revealed that the Beijing Han population clustered into the northern populations group and had a closer relationship with northern Han and Hui than with southern Han or other ethnic minorities. These results thus provide useful information that can be used in anthropology, selection for bone marrow transplantation as well as in disease-association study, such as in carbamazepine (CBZ)-induced Stevens-Johnson syndrome and toxic epidermal necrolysis.
为了鉴定中国北京汉族人群中 HLA-B15 亚型的分布，我们采用聚合酶链反应-序列基 因分型方法对 826 名无关的健康个体进行了分型。在 246 名 HLA-B15 阳性个体中，共鉴定出 29 种 HLA-B15 等位基因，其中最主要的是 B1501（40.07%），其次是 B1502（12.87%）、B1511（12.87%）、B1518（9.19%）和 B1532（3.31%）。我们比较了北京汉族人与其他 8 个中国少数民族以及涵盖中国大陆、台湾、香港和新加坡的 6 个人群中 HLA-B*15 亚型频率的分布情况。构建的邻接聚类系统发生树显示，北京汉族人群聚类到北方人群组，与北方汉族和回族的关系比与南方汉族或其他少数民族的关系更为密切。这些结果为人类学研究、骨髓移植供者选择以及疾病相关性研究（如卡马西平（CBZ）诱导的史蒂文斯-约翰逊综合征和中毒性表皮坏死松解症）提供了有用的信息。
