Mechanic S, Raynor K, Hill J E, Cowin P
Department of Cell Biology, New York University Medical Center, NY 10016.
Proc Natl Acad Sci U S A. 1991 May 15;88(10):4476-80. doi: 10.1073/pnas.88.10.4476.
The desmosomal adhesive core is formed by four major components: desmoglein (Mr, 165,000), desmocollins I and II (Mr, 120,000 and 110,000, respectively), and a Mr 22,000 protein. Here, we report the cloning and sequencing of cDNAs encoding a bovine desmocollin. The open reading frame found in the longest cDNA, 5 kilobases, contains a region encoding a protein of 839 amino acids. The features of the deduced amino acid sequence imply that the mature 707-amino acid desmocollin is a type I transmembrane protein that is produced by proteolytic cleavage of an 810-amino acid precursor. The ectodomain of desmocollin contains repeats that show extensive sequence similarity to members of the cadherin family of calcium-dependent cell adhesion molecules. A comparison of the amino acid sequences of desmocollin, desmoglein, and the cadherins shows that although these intercellular junctional adhesion molecules share a consensus sequence in their adhesive domains that defines them as a family, several features, including the divergence in the sequence of their cytoplasmic tails, divide them into three distinct subtypes.
桥粒芯糖蛋白（分子量165,000）、桥粒芯胶蛋白I和II（分子量分别为120,000和110,000）以及一种分子量22,000的蛋白质。在此，我们报道了编码牛桥粒芯胶蛋白的cDNA的克隆与测序。在最长的5千碱基cDNA中发现的开放阅读框包含一个编码839个氨基酸的蛋白质的区域。推导的氨基酸序列特征表明，成熟的707个氨基酸的桥粒芯胶蛋白是一种I型跨膜蛋白，由一个810个氨基酸的前体经蛋白水解切割产生。桥粒芯胶蛋白的胞外结构域含有一些重复序列，这些序列与钙依赖性细胞黏附分子钙黏蛋白家族的成员具有广泛的序列相似性。对桥粒芯胶蛋白、桥粒芯糖蛋白和钙黏蛋白的氨基酸序列比较表明，尽管这些细胞间连接黏附分子在其黏附结构域共享一个将它们定义为一个家族的共有序列，但包括其细胞质尾巴序列差异在内的几个特征将它们分为三个不同的亚型。
