Koch P J, Goldschmidt M D, Zimbelmann R, Troyanovsky R, Franke W W
Institute of Cell and Tumor Biology, German Cancer Research Center, Heidelberg.
Proc Natl Acad Sci U S A. 1992 Jan 1;89(1):353-7. doi: 10.1073/pnas.89.1.353.
Desmosomes are intercellular junctions that contain two major kinds of transmembrane glycoproteins, desmoglein and desmocollins I and II, involved in cell-cell adhesion. Recent sequence analyses have shown that both desmosomal glycoproteins belong to the larger cadherin family of cell adhesion molecules, in which they represent two different subgroups characterized by their specific sequence and topogenesis. In analyses of cDNA sequences and Northern blot experiments we have now found that both desmoglein and desmocollins are not unique gene products but occur in different subtypes produced from different genes. Comparison of the complete amino acid sequences of type 1 and type 2 desmocollins and of two desmoglein subtypes shows considerable divergence. While the desmoglein genes can be differentially expressed in different cell types, both type 1 and type 2 desmocollins can coexist in the same cells of certain stratified epithelia as shown by in situ hybridization. We conclude that the cadherin composition of desmosomes is much more complex than assumed and can differ in the various epithelia.
桥粒是一种细胞间连接结构，包含两种主要的跨膜糖蛋白，即桥粒芯糖蛋白和桥粒胶蛋白I及II，它们参与细胞间黏附。最近的序列分析表明，这两种桥粒糖蛋白都属于细胞黏附分子中较大的钙黏蛋白家族，在该家族中，它们代表两个不同的亚组，其特征在于特定的序列和拓扑结构。在对cDNA序列的分析和Northern印迹实验中，我们现在发现桥粒芯糖蛋白和桥粒胶蛋白都不是唯一的基因产物，而是由不同基因产生的不同亚型。1型和2型桥粒胶蛋白以及两种桥粒芯糖蛋白亚型的完整氨基酸序列比较显示出相当大的差异。虽然桥粒芯糖蛋白基因可以在不同细胞类型中差异表达，但原位杂交显示，1型和2型桥粒胶蛋白可以在某些复层上皮的同一细胞中共存。我们得出结论，桥粒的钙黏蛋白组成比预期的要复杂得多，并且在各种上皮中可能不同。
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