Institute of Animal Sciences, Physiology and Behaviour, ETH Zurich, CH-8092 Zurich, Switzerland.
Vet J. 2011 Apr;188(1):58-63. doi: 10.1016/j.tvjl.2010.02.012. Epub 2010 Mar 26.
The aim of the study was to evaluate bovine synoviocyte culture as an in vitro model to test new intra-articular drugs. The inflammatory reaction pattern of synoviocytes as compared to fibroblasts was studied over nine passages. Expression of pro-inflammatory cytokines was assessed after stimulation with lipopolysaccharide. Immunohistochemical markers were used to identify synoviocyte populations. Primary synoviocytes expressed markedly higher amounts of interleukin-1β mRNA and tumour necrosis factor-α mRNA than fibroblasts after stimulation. This difference was lost over two passages. CD68-positive macrophage-like synoviocytes diminished over three passages, which may explain the reduced pro-inflammatory cytokine response. Primary bovine synoviocytes appear to be an appropriate and optimised model for testing novel drugs for cattle, because their response may more closely reflect in vivo tissue responses compared to cultured cell lines.
本研究旨在评估牛滑膜细胞培养作为一种体外模型，以测试新的关节内药物。研究比较了滑膜细胞与成纤维细胞的炎症反应模式，共进行了九代培养。用脂多糖刺激后评估促炎细胞因子的表达。用免疫组织化学标志物鉴定滑膜细胞群体。刺激后，原代滑膜细胞表达的白细胞介素-1β mRNA 和肿瘤坏死因子-α mRNA 明显高于成纤维细胞。这种差异在两代后消失。CD68 阳性巨噬样滑膜细胞在三代后减少，这可能解释了促炎细胞因子反应的减少。与培养的细胞系相比，原代牛滑膜细胞似乎是一种合适的、优化的新型牛药物测试模型，因为它们的反应可能更接近体内组织反应。
