UMR S_763 Inserm / Université des Antilles et de la Guyane, Hôpital RICOU, CHU de Pointe-à-Pitre, 97159 Pointe-à-Pitre, Guadeloupe.
Clin Immunol. 2010 Jul;136(1):116-22. doi: 10.1016/j.clim.2010.02.023. Epub 2010 Mar 26.
Since inflammation plays a prominent role in the pathogenesis of sickle cell anemia (SCA) and Duffy antigen receptor for chemokines (DARC) modulates the function of inflammatory processes, we analyzed the relationship between the erythrocyte DARC phenotype and clinical expression of SCA. DARC locus was genotyped in 212 SS adult patients followed by the sickle cell center of Guadeloupe (French West Indies). After patients' stratification according to RBC DARC expression, the prevalence of renal disease, leg ulcers, priapism and osteonecrosis was compared between patient groups as well as hematological variables and plasma levels of chemokines. Duffy-positive patients exhibited higher counts of white blood cells (9.95+/-2.36 vs 8.88+/-2.32 10(9)/L, p=0.0066), polynuclear neutrophils (5.1+/-1.73 vs 4.51+/-1.71 10(9)/L, p=0.0227), higher plasma levels of IL-8 (4.46+/-1.22 vs 1.47+/-0.5 pg/mL, p=0.0202) and RANTES (27.8+/-4.3 vs 18.1+/-2.3 ng/mL, p=0.04) than Duffy-negative patients. No association was detected between RBC expression of DARC and the studied complications.
由于炎症在镰状细胞贫血症 (SCA) 的发病机制中起着重要作用，趋化因子受体 Duffy (DARC) 调节炎症过程的功能，我们分析了红细胞 DARC 表型与 SCA 临床表达之间的关系。在法国西印度群岛瓜德罗普岛的镰状细胞中心，对 212 例 SS 成年患者进行了 DARC 基因座基因型分析。对患者进行 RBC DARC 表达分层后，比较了两组患者的肾脏疾病、腿部溃疡、阴茎异常勃起和骨坏死的患病率，以及血液学变量和趋化因子的血浆水平。Duffy 阳性患者的白细胞计数更高（9.95+/-2.36 与 8.88+/-2.32 10(9)/L，p=0.0066）、多核中性粒细胞计数更高（5.1+/-1.73 与 4.51+/-1.71 10(9)/L，p=0.0227）、白细胞介素 8（IL-8）的血浆水平更高（4.46+/-1.22 与 1.47+/-0.5 pg/mL，p=0.0202）和 RANTES（27.8+/-4.3 与 18.1+/-2.3 ng/mL，p=0.04），而非 Duffy 阴性患者。未检测到 RBC DARC 表达与研究并发症之间存在关联。
